Structural Analysis of 2D-Gel-Separated Glycoproteins from Human Cerebrospinal Fluid by Tandem High-Resolution Mass Spectrometry

Kristina Håkansson; Mark R. Emmett; Alan G. Marshall; Pia Davidsson; Carol L. Nilsson

doi:10.1021/pr034030n

Structural Analysis of 2D-Gel-Separated Glycoproteins from Human Cerebrospinal Fluid by Tandem High-Resolution Mass Spectrometry

Kristina Håkansson, Mark R. Emmett, Alan G. Marshall, Pia Davidsson, Carol L. Nilsson

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

The feasibility of global glycoprotein analysis by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and infrared multiphoton dissociation (IRMPD) tandem mass spectrometry is demonstrated. Combined 2D gel glycoprotein separation and visualization, in-gel digestion, and accurate (<10 ppm) mass measurement allowed identification of human glycoproteins and revealed differences in glycosylation. IRMPD obviates the need for glycan release, which prevents sample dispersal, and allows the assignment of glycan structures to specific sites of N-glycosylation.

Original language	English (US)
Pages (from-to)	581-588
Number of pages	8
Journal	Journal of Proteome Research
Volume	2
Issue number	6
DOIs	https://doi.org/10.1021/pr034030n
State	Published - Nov 2003
Externally published	Yes

Keywords

2D-PAGE
CSF
Cerebrospinal fluid
ESI
Electrospray
FT-ICR
FTMS
Fourier transform
Glycoprotein
Glycoproteomics
Glycosylation
IRMPD
Infrared multiphoton dissociation
Ion cyclotron resonance
MS/MS
Proteomics
Tandem MS
α-1-antitrypsin
β-trace

ASJC Scopus subject areas

General Chemistry
Biochemistry

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10.1021/pr034030n

Cite this

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title = "Structural Analysis of 2D-Gel-Separated Glycoproteins from Human Cerebrospinal Fluid by Tandem High-Resolution Mass Spectrometry",

abstract = "The feasibility of global glycoprotein analysis by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and infrared multiphoton dissociation (IRMPD) tandem mass spectrometry is demonstrated. Combined 2D gel glycoprotein separation and visualization, in-gel digestion, and accurate (<10 ppm) mass measurement allowed identification of human glycoproteins and revealed differences in glycosylation. IRMPD obviates the need for glycan release, which prevents sample dispersal, and allows the assignment of glycan structures to specific sites of N-glycosylation.",

keywords = "2D-PAGE, CSF, Cerebrospinal fluid, ESI, Electrospray, FT-ICR, FTMS, Fourier transform, Glycoprotein, Glycoproteomics, Glycosylation, IRMPD, Infrared multiphoton dissociation, Ion cyclotron resonance, MS/MS, Proteomics, Tandem MS, α-1-antitrypsin, β-trace",

author = "Kristina H{\aa}kansson and Emmett, {Mark R.} and Marshall, {Alan G.} and Pia Davidsson and Nilsson, {Carol L.}",

year = "2003",

month = nov,

doi = "10.1021/pr034030n",

language = "English (US)",

volume = "2",

pages = "581--588",

journal = "Journal of Proteome Research",

issn = "1535-3893",

publisher = "American Chemical Society",

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TY - JOUR

T1 - Structural Analysis of 2D-Gel-Separated Glycoproteins from Human Cerebrospinal Fluid by Tandem High-Resolution Mass Spectrometry

AU - Håkansson, Kristina

AU - Emmett, Mark R.

AU - Marshall, Alan G.

AU - Davidsson, Pia

AU - Nilsson, Carol L.

PY - 2003/11

Y1 - 2003/11

N2 - The feasibility of global glycoprotein analysis by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and infrared multiphoton dissociation (IRMPD) tandem mass spectrometry is demonstrated. Combined 2D gel glycoprotein separation and visualization, in-gel digestion, and accurate (<10 ppm) mass measurement allowed identification of human glycoproteins and revealed differences in glycosylation. IRMPD obviates the need for glycan release, which prevents sample dispersal, and allows the assignment of glycan structures to specific sites of N-glycosylation.

AB - The feasibility of global glycoprotein analysis by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and infrared multiphoton dissociation (IRMPD) tandem mass spectrometry is demonstrated. Combined 2D gel glycoprotein separation and visualization, in-gel digestion, and accurate (<10 ppm) mass measurement allowed identification of human glycoproteins and revealed differences in glycosylation. IRMPD obviates the need for glycan release, which prevents sample dispersal, and allows the assignment of glycan structures to specific sites of N-glycosylation.

KW - 2D-PAGE

KW - CSF

KW - Cerebrospinal fluid

KW - ESI

KW - Electrospray

KW - FT-ICR

KW - FTMS

KW - Fourier transform

KW - Glycoprotein

KW - Glycoproteomics

KW - Glycosylation

KW - IRMPD

KW - Infrared multiphoton dissociation

KW - Ion cyclotron resonance

KW - MS/MS

KW - Proteomics

KW - Tandem MS

KW - α-1-antitrypsin

KW - β-trace

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U2 - 10.1021/pr034030n

DO - 10.1021/pr034030n

M3 - Article

C2 - 14692451

AN - SCOPUS:0348142992

SN - 1535-3893

VL - 2

SP - 581

EP - 588

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 6

ER -

Structural Analysis of 2D-Gel-Separated Glycoproteins from Human Cerebrospinal Fluid by Tandem High-Resolution Mass Spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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