Structural Analysis of 2D-Gel-Separated Glycoproteins from Human Cerebrospinal Fluid by Tandem High-Resolution Mass Spectrometry

Kristina Håkansson, Mark Emmett, Alan G. Marshall, Pia Davidsson, Carol L. Nilsson

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The feasibility of global glycoprotein analysis by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and infrared multiphoton dissociation (IRMPD) tandem mass spectrometry is demonstrated. Combined 2D gel glycoprotein separation and visualization, in-gel digestion, and accurate (<10 ppm) mass measurement allowed identification of human glycoproteins and revealed differences in glycosylation. IRMPD obviates the need for glycan release, which prevents sample dispersal, and allows the assignment of glycan structures to specific sites of N-glycosylation.

Original languageEnglish (US)
Pages (from-to)581-588
Number of pages8
JournalJournal of Proteome Research
Volume2
Issue number6
DOIs
StatePublished - Nov 2003
Externally publishedYes

Fingerprint

Cerebrospinal fluid
Structural analysis
Glycosylation
Mass spectrometry
Cerebrospinal Fluid
Mass Spectrometry
Glycoproteins
Gels
Polysaccharides
Infrared radiation
Cyclotrons
Cyclotron resonance
Electrospray ionization
Forensic Anthropology
Fourier Analysis
Tandem Mass Spectrometry
Digestion
Fourier transforms
Visualization
Ions

Keywords

  • α-1-antitrypsin
  • β-trace
  • 2D-PAGE
  • Cerebrospinal fluid
  • CSF
  • Electrospray
  • ESI
  • Fourier transform
  • FT-ICR
  • FTMS
  • Glycoprotein
  • Glycoproteomics
  • Glycosylation
  • Infrared multiphoton dissociation
  • Ion cyclotron resonance
  • IRMPD
  • MS/MS
  • Proteomics
  • Tandem MS

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

Cite this

Structural Analysis of 2D-Gel-Separated Glycoproteins from Human Cerebrospinal Fluid by Tandem High-Resolution Mass Spectrometry. / Håkansson, Kristina; Emmett, Mark; Marshall, Alan G.; Davidsson, Pia; Nilsson, Carol L.

In: Journal of Proteome Research, Vol. 2, No. 6, 11.2003, p. 581-588.

Research output: Contribution to journalArticle

Håkansson, Kristina ; Emmett, Mark ; Marshall, Alan G. ; Davidsson, Pia ; Nilsson, Carol L. / Structural Analysis of 2D-Gel-Separated Glycoproteins from Human Cerebrospinal Fluid by Tandem High-Resolution Mass Spectrometry. In: Journal of Proteome Research. 2003 ; Vol. 2, No. 6. pp. 581-588.
@article{650cb360be134c94a5d5ec49aec8dac7,
title = "Structural Analysis of 2D-Gel-Separated Glycoproteins from Human Cerebrospinal Fluid by Tandem High-Resolution Mass Spectrometry",
abstract = "The feasibility of global glycoprotein analysis by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and infrared multiphoton dissociation (IRMPD) tandem mass spectrometry is demonstrated. Combined 2D gel glycoprotein separation and visualization, in-gel digestion, and accurate (<10 ppm) mass measurement allowed identification of human glycoproteins and revealed differences in glycosylation. IRMPD obviates the need for glycan release, which prevents sample dispersal, and allows the assignment of glycan structures to specific sites of N-glycosylation.",
keywords = "α-1-antitrypsin, β-trace, 2D-PAGE, Cerebrospinal fluid, CSF, Electrospray, ESI, Fourier transform, FT-ICR, FTMS, Glycoprotein, Glycoproteomics, Glycosylation, Infrared multiphoton dissociation, Ion cyclotron resonance, IRMPD, MS/MS, Proteomics, Tandem MS",
author = "Kristina H{\aa}kansson and Mark Emmett and Marshall, {Alan G.} and Pia Davidsson and Nilsson, {Carol L.}",
year = "2003",
month = "11",
doi = "10.1021/pr034030n",
language = "English (US)",
volume = "2",
pages = "581--588",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "6",

}

TY - JOUR

T1 - Structural Analysis of 2D-Gel-Separated Glycoproteins from Human Cerebrospinal Fluid by Tandem High-Resolution Mass Spectrometry

AU - Håkansson, Kristina

AU - Emmett, Mark

AU - Marshall, Alan G.

AU - Davidsson, Pia

AU - Nilsson, Carol L.

PY - 2003/11

Y1 - 2003/11

N2 - The feasibility of global glycoprotein analysis by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and infrared multiphoton dissociation (IRMPD) tandem mass spectrometry is demonstrated. Combined 2D gel glycoprotein separation and visualization, in-gel digestion, and accurate (<10 ppm) mass measurement allowed identification of human glycoproteins and revealed differences in glycosylation. IRMPD obviates the need for glycan release, which prevents sample dispersal, and allows the assignment of glycan structures to specific sites of N-glycosylation.

AB - The feasibility of global glycoprotein analysis by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and infrared multiphoton dissociation (IRMPD) tandem mass spectrometry is demonstrated. Combined 2D gel glycoprotein separation and visualization, in-gel digestion, and accurate (<10 ppm) mass measurement allowed identification of human glycoproteins and revealed differences in glycosylation. IRMPD obviates the need for glycan release, which prevents sample dispersal, and allows the assignment of glycan structures to specific sites of N-glycosylation.

KW - α-1-antitrypsin

KW - β-trace

KW - 2D-PAGE

KW - Cerebrospinal fluid

KW - CSF

KW - Electrospray

KW - ESI

KW - Fourier transform

KW - FT-ICR

KW - FTMS

KW - Glycoprotein

KW - Glycoproteomics

KW - Glycosylation

KW - Infrared multiphoton dissociation

KW - Ion cyclotron resonance

KW - IRMPD

KW - MS/MS

KW - Proteomics

KW - Tandem MS

UR - http://www.scopus.com/inward/record.url?scp=0348142992&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0348142992&partnerID=8YFLogxK

U2 - 10.1021/pr034030n

DO - 10.1021/pr034030n

M3 - Article

VL - 2

SP - 581

EP - 588

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 6

ER -