Structural Analysis of 2D-Gel-Separated Glycoproteins from Human Cerebrospinal Fluid by Tandem High-Resolution Mass Spectrometry

Kristina Håkansson, Mark Emmett, Alan G. Marshall, Pia Davidsson, Carol L. Nilsson

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

The feasibility of global glycoprotein analysis by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and infrared multiphoton dissociation (IRMPD) tandem mass spectrometry is demonstrated. Combined 2D gel glycoprotein separation and visualization, in-gel digestion, and accurate (<10 ppm) mass measurement allowed identification of human glycoproteins and revealed differences in glycosylation. IRMPD obviates the need for glycan release, which prevents sample dispersal, and allows the assignment of glycan structures to specific sites of N-glycosylation.

Original languageEnglish (US)
Pages (from-to)581-588
Number of pages8
JournalJournal of Proteome Research
Volume2
Issue number6
DOIs
StatePublished - Nov 2003
Externally publishedYes

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Keywords

  • α-1-antitrypsin
  • β-trace
  • 2D-PAGE
  • Cerebrospinal fluid
  • CSF
  • Electrospray
  • ESI
  • Fourier transform
  • FT-ICR
  • FTMS
  • Glycoprotein
  • Glycoproteomics
  • Glycosylation
  • Infrared multiphoton dissociation
  • Ion cyclotron resonance
  • IRMPD
  • MS/MS
  • Proteomics
  • Tandem MS

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

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