Structural analysis of adult and larval isozymes of sn-glycerol-3-phosphate dehydrogenase of Drosophila melanogaster.

David Niesel, Y. C. Pan, G. C. Bewley, F. B. Armstrong, S. S. Li

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Compositional analysis of the soluble tryptic peptides representing about 70% of the 293 residues of sn-glycerol-3-phosphate dehydrogenase in Drosophila melanogaster reveals a single peptide difference between the sn-glycerol-3-phosphate dehydrogenase adult (GPDHF-1) and larval (GPDHF-3) isozymes. This peptide was shown to be the carboxyl terminus by sequence determination and by carboxypeptidase A digestion of the native protein. For GPDHF-1, the sequence of the COOH-terminal tryptic peptide is Asn-His-Pro-Glu-His-Met-Gln-Asn-Leu-COOH, while that of GPDHF-3 is Asn-His-Pro-Glu-His-Met-COOH.

Original languageEnglish (US)
Pages (from-to)979-983
Number of pages5
JournalJournal of Biological Chemistry
Volume257
Issue number2
StatePublished - Jan 25 1982
Externally publishedYes

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Glycerolphosphate Dehydrogenase
Drosophila melanogaster
Structural analysis
Isoenzymes
Peptides
Carboxypeptidases A
Proteolysis
Sequence Analysis
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Structural analysis of adult and larval isozymes of sn-glycerol-3-phosphate dehydrogenase of Drosophila melanogaster. / Niesel, David; Pan, Y. C.; Bewley, G. C.; Armstrong, F. B.; Li, S. S.

In: Journal of Biological Chemistry, Vol. 257, No. 2, 25.01.1982, p. 979-983.

Research output: Contribution to journalArticle

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