Structural analysis of invariant chain subsets as a function of their association with MHC Class II Chains

Quoc V. Nguyen, Victor E. Reyes, Robert E. Humphreys

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Respective subsets of human invariant chain (Ii), as identified with antibodies to two different epitopes, were characterized as a function of their associations with major histocompatibility complex (MHC) class II α, β chains and intracellular processing. E1 antiserum to Ii(183-193) and VIC-Y1 monoclonal antibody to an N-terminal determinant identified Ii(E1) and Ii(VIC) populations, respectively. Ii proteins comprise several species which have been defined with either genomic or post-translational processes: Ii itself; IpN and IpO, which represent the glycosylated forms on asparagine or threonine/serine, respectively; γ2 and γ3, which originate from an alternative initiation site for transcription; and p41, which has a 64-amino-acid insert which originated from an additional exon placed after the sixth exon of Ii. Immunoprecipitates of detergent-solubilized protein complexes from [35S]methionine-labeled Raji cells showed that Ii(E1) consisted of Ii, p41, IpN, and immature α chain, while Ii(VIC) consisted of Ii, processed Ii with N- and O-linked glycosylation (IpN, IpO), p41, and associated MHC class II α, β chains. In the MHC class II-deficient P3HR-1 cells, Ii(E1) and Ii(VIC) were virtually identical. Ii(E1) was resistant to cathepsin B digestion while Ii(VIC) was sensitive. In pulse-chase radiolabeling experiments with brefeldin A (BFA)-treated cells, II(VIC) progressively became resistant to endoglycosidase H (endo H) and had a longer half-life than that in cells not treated with BFA, but Ii(E1) remained susceptible to endo H and its half-life was unaffected by BFA. Since BFA redistributes Golgi enzymes to the endoplasmic reticulum, this observation suggests that H(E1) is protected from processing enzymes while Ii(VIC) is not. These studies define association of Ii with MHC class II molecules when certain epitopes on Ii are exposed or not. These differences relate to intracellular transport of Ii and to its release for the binding of antigenic peptides.

Original languageEnglish (US)
Pages (from-to)128-132
Number of pages5
JournalArchives of Biochemistry and Biophysics
Volume317
Issue number1
DOIs
StatePublished - Feb 20 1995

Keywords

  • Brefeldin
  • Epitope
  • Glycosylation
  • Ii
  • Teolysis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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