Structural and dynamic properties of a bromouracil-adenine base pair in DNA studied by proton NMR

G. V. Fazakerley, Lawrence Sowers, R. Eritja, B. E. Kaplan, M. F. Goodman

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We have synthesized and studied by proton NMR a duplex heptaoligonucleotide containing a 5-bromouracil (brU)-adenine base pair. This represents the first structural characterization of a B-form DNA containing brU. The brU·A base pair is Watson-Crick rather than Hoogsteen as seen for the monomers in the crystalline state. From analysis of the NOESY spectra at very short mixing times evidence is presented that substitution of brU for T induces significant conformational changes from that of a normal B DNA. The helix twist between brU4·A11 and G3·C12 is ca. 15° and for both brU4 and G3 the glycosyl torsion angles are significantly changed. The imino proton of the bru·A base pair shows a pH insensitive line width which shows that the pK of brU in this base pair is very much higher than that of the monomer.

Original languageEnglish (US)
Pages (from-to)639-650
Number of pages12
JournalJournal of Biomolecular Structure and Dynamics
Volume5
Issue number3
StatePublished - 1987
Externally publishedYes

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Bromouracil
Adenine
Base Pairing
Protons
DNA
B-Form DNA
Spectrum Analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

Structural and dynamic properties of a bromouracil-adenine base pair in DNA studied by proton NMR. / Fazakerley, G. V.; Sowers, Lawrence; Eritja, R.; Kaplan, B. E.; Goodman, M. F.

In: Journal of Biomolecular Structure and Dynamics, Vol. 5, No. 3, 1987, p. 639-650.

Research output: Contribution to journalArticle

Fazakerley, G. V. ; Sowers, Lawrence ; Eritja, R. ; Kaplan, B. E. ; Goodman, M. F. / Structural and dynamic properties of a bromouracil-adenine base pair in DNA studied by proton NMR. In: Journal of Biomolecular Structure and Dynamics. 1987 ; Vol. 5, No. 3. pp. 639-650.
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AU - Goodman, M. F.

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