Structural and dynamic properties of a bromouracil-adenine base pair in dna studied by proton nmr

G. Victor Fazakerley, Lawrence C. Sowers, Ramon Eritja, Bruce E. Kaplan, Myron F. Goodman

Research output: Contribution to journalArticle

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Abstract

We have synthesized and studied by proton NMR a duplex heptaoligonucleotide containing a 5-bromouracil (brU) o adenine base pair. This represents the first structural characterization of a B-form DNA containing brU. The brU · A base pair is Watson-Crick rather than Hoogsteen as seen for the monomers in the crystalline state. From analysis of the NOESY sepctra at very short mixing times evidence is presented that substitution of brU for T induces significant conformational changes from that of a normal B DNA The helix twist between brU4 · All and G3 · Cl2 isca.l5o and for both brU4and G3 the glycosyltorsion angles are significantly changed. The imino proton of the bru ·A base pair shows a pH insensitive line width which shows that the pK of brU in this base pair is very much higher than that of the monomer.

Original languageEnglish (US)
Pages (from-to)639-650
Number of pages12
JournalJournal of Biomolecular Structure and Dynamics
Volume5
Issue number3
DOIs
StatePublished - Dec 1987
Externally publishedYes

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ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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