Structural and dynamic properties of a bromouracil-adenine base pair in dna studied by proton nmr

G. Victor Fazakerley; Lawrence C. Sowers; Ramon Eritja; Bruce E. Kaplan; Myron F. Goodman

doi:10.1080/07391102.1987.10506417

Structural and dynamic properties of a bromouracil-adenine base pair in dna studied by proton nmr

G. Victor Fazakerley, Lawrence C. Sowers, Ramon Eritja, Bruce E. Kaplan, Myron F. Goodman

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

We have synthesized and studied by proton NMR a duplex heptaoligonucleotide containing a 5-bromouracil (brU) o adenine base pair. This represents the first structural characterization of a B-form DNA containing brU. The brU · A base pair is Watson-Crick rather than Hoogsteen as seen for the monomers in the crystalline state. From analysis of the NOESY sepctra at very short mixing times evidence is presented that substitution of brU for T induces significant conformational changes from that of a normal B DNA The helix twist between brU4 · All and G3 · Cl2 isca.l5o and for both brU4and G3 the glycosyltorsion angles are significantly changed. The imino proton of the bru ·A base pair shows a pH insensitive line width which shows that the pK of brU in this base pair is very much higher than that of the monomer.

Original language	English (US)
Pages (from-to)	639-650
Number of pages	12
Journal	Journal of Biomolecular Structure and Dynamics
Volume	5
Issue number	3
DOIs	https://doi.org/10.1080/07391102.1987.10506417
State	Published - Dec 1987
Externally published	Yes

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1080/07391102.1987.10506417

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title = "Structural and dynamic properties of a bromouracil-adenine base pair in dna studied by proton nmr",

abstract = "We have synthesized and studied by proton NMR a duplex heptaoligonucleotide containing a 5-bromouracil (brU) o adenine base pair. This represents the first structural characterization of a B-form DNA containing brU. The brU · A base pair is Watson-Crick rather than Hoogsteen as seen for the monomers in the crystalline state. From analysis of the NOESY sepctra at very short mixing times evidence is presented that substitution of brU for T induces significant conformational changes from that of a normal B DNA The helix twist between brU4 · All and G3 · Cl2 isca.l5o and for both brU4and G3 the glycosyltorsion angles are significantly changed. The imino proton of the bru ·A base pair shows a pH insensitive line width which shows that the pK of brU in this base pair is very much higher than that of the monomer.",

author = "Fazakerley, {G. Victor} and Sowers, {Lawrence C.} and Ramon Eritja and Kaplan, {Bruce E.} and Goodman, {Myron F.}",

note = "Funding Information: This research was supported by National Institutes ofHealth Grants GM 33863 and G M 21422. L. S. was supported by NRSA 2T32CA0932004 from theN ational Cancer Institute.",

year = "1987",

month = dec,

doi = "10.1080/07391102.1987.10506417",

language = "English (US)",

volume = "5",

pages = "639--650",

journal = "Journal of Biomolecular Structure and Dynamics",

issn = "0739-1102",

publisher = "Taylor and Francis Ltd.",

number = "3",

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T1 - Structural and dynamic properties of a bromouracil-adenine base pair in dna studied by proton nmr

AU - Fazakerley, G. Victor

AU - Sowers, Lawrence C.

AU - Eritja, Ramon

AU - Kaplan, Bruce E.

AU - Goodman, Myron F.

N1 - Funding Information: This research was supported by National Institutes ofHealth Grants GM 33863 and G M 21422. L. S. was supported by NRSA 2T32CA0932004 from theN ational Cancer Institute.

PY - 1987/12

Y1 - 1987/12

N2 - We have synthesized and studied by proton NMR a duplex heptaoligonucleotide containing a 5-bromouracil (brU) o adenine base pair. This represents the first structural characterization of a B-form DNA containing brU. The brU · A base pair is Watson-Crick rather than Hoogsteen as seen for the monomers in the crystalline state. From analysis of the NOESY sepctra at very short mixing times evidence is presented that substitution of brU for T induces significant conformational changes from that of a normal B DNA The helix twist between brU4 · All and G3 · Cl2 isca.l5o and for both brU4and G3 the glycosyltorsion angles are significantly changed. The imino proton of the bru ·A base pair shows a pH insensitive line width which shows that the pK of brU in this base pair is very much higher than that of the monomer.

AB - We have synthesized and studied by proton NMR a duplex heptaoligonucleotide containing a 5-bromouracil (brU) o adenine base pair. This represents the first structural characterization of a B-form DNA containing brU. The brU · A base pair is Watson-Crick rather than Hoogsteen as seen for the monomers in the crystalline state. From analysis of the NOESY sepctra at very short mixing times evidence is presented that substitution of brU for T induces significant conformational changes from that of a normal B DNA The helix twist between brU4 · All and G3 · Cl2 isca.l5o and for both brU4and G3 the glycosyltorsion angles are significantly changed. The imino proton of the bru ·A base pair shows a pH insensitive line width which shows that the pK of brU in this base pair is very much higher than that of the monomer.

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JO - Journal of Biomolecular Structure and Dynamics

JF - Journal of Biomolecular Structure and Dynamics

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Structural and dynamic properties of a bromouracil-adenine base pair in dna studied by proton nmr

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