Structural and functional conservation of two human homologs of the yeast DNA repair gene RAD6

Marcel H.M. Koken, Paul Reynolds, Iris Jaspers-Dekker, Louise Prakash, Satya Prakash, Dirk Bootsma, Jan H.J. Hoeijmakers

Research output: Contribution to journalArticlepeer-review

214 Scopus citations

Abstract

The RAD6 gene of Saccharomyces cerevisiae encodes a ubiquitin-conjugating enzyme (E2) that is required for DNA repair, damage-induced mutagenesis, and sporulation. We have cloned the two human RAD6 homologs, designated HHR6A and HHR6B. The two 152-amino acid human proteins share 95% sequence identity with each other and ≈70% and ≈85% overall identity with the homologs from yeasts (S. cerevisiae and Schizosaccharomyces pombe) and Drosophila melanogaster, respectively. Neither of the human RAD6 homologs possesses the acidic C-terminal sequence present in the S. cerevisiae RAD6 protein. Genetic complementation experiments reveal that HHR6A as well as HHR6B can carry out the DNA repair and mutagenesis functions of RAD6 in S. cerevisiae rad6Δ mutants.

Original languageEnglish (US)
Pages (from-to)8865-8869
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number20
DOIs
StatePublished - Oct 15 1991

Keywords

  • DNA damage
  • E2 enzyme
  • Sporulation
  • UV mutagenesis
  • Ubiquitin conjugation

ASJC Scopus subject areas

  • General

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