Structural and functional similarities between the capsid proteins of bacteriophages T4 and HK97 point to a common ancestry

Andrei Fokine, Petr Leiman, Mikhail M. Shneider, Bijan Ahvazi, Karen M. Boeshans, Alasdair C. Steven, Lindsay W. Black, Vadim V. Mesyanzhinov, Michael G. Rossmann

Research output: Contribution to journalArticle

146 Citations (Scopus)

Abstract

Gene product (gp) 24 of bacteriophage T4 forms the pentameric vertices of the capsid. Using x-ray crystallography, we found the principal domain of gp24 to have a polypeptide fold similar to that of the HK97 phage capsid protein plus an additional insertion domain. Fitting gp24 monomers into a cryo-EM density map of the mature T4 capsid suggests that the insertion domain interacts with a neighboring subunit, effecting a stabilization analogous to the covalent crosslinking in the HK97 capsid. Sequence alignment and genetic data show that the folds of gp24 and the hexamer-forming capsid protein, gp23*, are similar. Accordingly, models of gp24* pentamers, gp23* hexamers, and the whole capsid were built, based on a cryo-EM image reconstruction of the capsid. Mutations in gene 23 that affect capsid shape map to the capsomer's periphery, whereas mutations that allow gp23 to substitute for gp24 at the vertices modify the interactions between monomers within capsomers. Structural data show that capsid proteins of most tailed phages, and some eukaryotic viruses, may have evolved from a common ancestor.

Original languageEnglish (US)
Pages (from-to)7163-7168
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number20
DOIs
StatePublished - May 17 2005
Externally publishedYes

Fingerprint

Bacteriophage T4
Capsid
Capsid Proteins
Bacteriophages
Crystallography
Mutation
Computer-Assisted Image Processing
Sequence Alignment
Genes
X-Rays
Viruses
Peptides

Keywords

  • Evolution
  • Gene product 24
  • Major capsid protein

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Structural and functional similarities between the capsid proteins of bacteriophages T4 and HK97 point to a common ancestry. / Fokine, Andrei; Leiman, Petr; Shneider, Mikhail M.; Ahvazi, Bijan; Boeshans, Karen M.; Steven, Alasdair C.; Black, Lindsay W.; Mesyanzhinov, Vadim V.; Rossmann, Michael G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 20, 17.05.2005, p. 7163-7168.

Research output: Contribution to journalArticle

Fokine, Andrei ; Leiman, Petr ; Shneider, Mikhail M. ; Ahvazi, Bijan ; Boeshans, Karen M. ; Steven, Alasdair C. ; Black, Lindsay W. ; Mesyanzhinov, Vadim V. ; Rossmann, Michael G. / Structural and functional similarities between the capsid proteins of bacteriophages T4 and HK97 point to a common ancestry. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 20. pp. 7163-7168.
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