Structural and Molecular Basis for Coordination in a Viral DNA Packaging Motor

Huzhang Mao, Mitul Saha, Emilio Reyes-Aldrete, Michael Sherman, Michael Woodson, Rockney Atz, Shelley Grimes, Paul J. Jardine, Marc Morais

    Research output: Contribution to journalArticle

    25 Citations (Scopus)

    Abstract

    Ring NTPases are a class of ubiquitous molecular motors involved in basic biological partitioning processes. dsDNA viruses encode ring ATPases that translocate their genomes to near-crystalline densities within pre-assembled viral capsids. Here, X-ray crystallography, cryoEM, and biochemical analyses of the dsDNA packaging motor in bacteriophage phi29 show how individual subunits are arranged in a pentameric ATPase ring and suggest how their activities are coordinated to translocate dsDNA. The resulting pseudo-atomic structure of the motor and accompanying functional analyses show how ATP is bound in the ATPase active site; identify two DNA contacts, including a potential DNA translocating loop; demonstrate that a trans-acting arginine finger is involved in coordinating hydrolysis around the ring; and suggest a functional coupling between the arginine finger and the DNA translocating loop. The ability to visualize the motor in action illuminates how the different motor components interact with each other and with their DNA substrate.

    Original languageEnglish (US)
    Pages (from-to)2017-2029
    Number of pages13
    JournalCell Reports
    Volume14
    Issue number8
    DOIs
    StatePublished - Mar 1 2016

    Fingerprint

    DNA Packaging
    Virus Assembly
    Viral DNA
    Packaging
    Adenosine Triphosphatases
    DNA
    Fingers
    Arginine
    Biological Phenomena
    Capsid
    X Ray Crystallography
    Product Packaging
    Bacteriophages
    X ray crystallography
    Catalytic Domain
    Hydrolysis
    Viruses
    Adenosine Triphosphate
    Genome
    Genes

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

    Cite this

    Structural and Molecular Basis for Coordination in a Viral DNA Packaging Motor. / Mao, Huzhang; Saha, Mitul; Reyes-Aldrete, Emilio; Sherman, Michael; Woodson, Michael; Atz, Rockney; Grimes, Shelley; Jardine, Paul J.; Morais, Marc.

    In: Cell Reports, Vol. 14, No. 8, 01.03.2016, p. 2017-2029.

    Research output: Contribution to journalArticle

    Mao, H, Saha, M, Reyes-Aldrete, E, Sherman, M, Woodson, M, Atz, R, Grimes, S, Jardine, PJ & Morais, M 2016, 'Structural and Molecular Basis for Coordination in a Viral DNA Packaging Motor', Cell Reports, vol. 14, no. 8, pp. 2017-2029. https://doi.org/10.1016/j.celrep.2016.01.058
    Mao, Huzhang ; Saha, Mitul ; Reyes-Aldrete, Emilio ; Sherman, Michael ; Woodson, Michael ; Atz, Rockney ; Grimes, Shelley ; Jardine, Paul J. ; Morais, Marc. / Structural and Molecular Basis for Coordination in a Viral DNA Packaging Motor. In: Cell Reports. 2016 ; Vol. 14, No. 8. pp. 2017-2029.
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