Structural basis for sensory rhodopsin function

Eva Pebay-Peyroula, Antoine Royant, Ehud M. Landau, Javier Navarro

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The crystal structure of sensory rhodopsin II from Natronobacterium pharaonis was recently solved at 2.1 Å resolution from lipidic cubic phase-grown crystals. A critical analysis of previous structure-function studies is possible within the framework of the high-resolution structure of this photoreceptor. Based on the structure, a molecular understanding emerges of the efficiency and selectivity of the photoisomerization reaction, of the interaction of the sensory receptor and its cognate transducer protein HtrII, and of the mechanism of spectral tuning in photoreceptors. The architecture of the retinal binding pocket is compact, representing a major determinant for the selective binding of the chromophore, all-trans retinal to the apoprotein, opsin. Several chromophore-protein interactions revealed by the structure were not predicted by previous mutagenesis and spectroscopic analyses. The structure suggests likely mechanisms by which photoisomerization triggers the activation of sensory rhodopsin II, and highlights the possibility of a unified mechanism of signaling mediated by sensory receptors, including visual rhodopsins. Future investigations using time-resolved crystallography, structural dynamics, and computational studies will provide the basis to unveil the molecular mechanisms of sensory receptors-mediated transmembrane signaling.

Original languageEnglish (US)
Pages (from-to)196-205
Number of pages10
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1565
Issue number2
DOIs
StatePublished - Oct 11 2002
Externally publishedYes

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Sensory Rhodopsins
Photoisomerization
Sensory Receptor Cells
Chromophores
Opsins
Mutagenesis
Apoproteins
Rhodopsin
Crystallography
Natronobacterium
Structural dynamics
Transducers
Proteins
Tuning
Crystal structure
Chemical activation
Molecular Structure
Crystals

Keywords

  • Color tuning
  • Crystallography
  • Sensory rhodopsin
  • Signal transduction
  • Structural mechanism

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

Cite this

Structural basis for sensory rhodopsin function. / Pebay-Peyroula, Eva; Royant, Antoine; Landau, Ehud M.; Navarro, Javier.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1565, No. 2, 11.10.2002, p. 196-205.

Research output: Contribution to journalArticle

Pebay-Peyroula, Eva ; Royant, Antoine ; Landau, Ehud M. ; Navarro, Javier. / Structural basis for sensory rhodopsin function. In: Biochimica et Biophysica Acta - Biomembranes. 2002 ; Vol. 1565, No. 2. pp. 196-205.
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