Structural basis for the activity of pp60c-src protein tyrosine kinase inhibitors

Ninad V. Prabhu, Subeeh A. Siddiqui, John S. McMurray, B. Montgomery Pettitt

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Conformational searches on three closely related pp60c-src protein tyrosine kinase inhibitors of varying potencies were performed to determine a structural basis for their activity. The first was a linear peptide (PDNEYAFFQf), the second its 10-membered cyclic analogue, and the third a cyclic analogue with a para carboxyphenylalanine in place of one the F residues. A common backbone conformation with an antiparallel β-sheet-like geometry capped by similar β-turns was found for all three peptides, which may be a binding conformation and gives a candidate pharmacophore for further testing. The interaction between some polar side chains and between some of the aromatic rings may be important for maintaining the correct conformation. The differences in potencies of these inhibitors may be attributed to certain thermodynamic and chemical reasons.

Original languageEnglish (US)
Pages (from-to)167-179
Number of pages13
JournalBiopolymers
Volume59
Issue number3
DOIs
StatePublished - Aug 11 2001
Externally publishedYes

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Keywords

  • Antiparallel β-sheet
  • Backbone conformation
  • Cyclic analogue
  • Linear peptide
  • pp protein tyrosine kinase inhibitors
  • β-turn

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Biomaterials
  • Organic Chemistry

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