Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase

Y. Whitney Yin, Thomas A. Steitz

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

To make messenger RNA transcripts, bacteriophage T7 RNA polymerase (T7 RNAP) undergoes a transition from an initiation phase, which only makes short RNA fragments, to a stable elongation phase. We have determined at 2.1 angstrom resolution the crystal structure of a T7 RNAP elongation complex with 30 base pairs of duplex DNA containing a “transcription bubble” interacting with a 17-nucleotide RNA transcript. The transition from an initiation to an elongation complex is accompanied by a major refolding of the amino-terminal 300 residues. This results in loss of the promoter binding site, facilitating promoter clearance, and creates a tunnel that surrounds the RNA transcript after it peels off a seven– base pair heteroduplex. Formation of the exit tunnel explains the enhanced processivity of the elongation complex. Downstream duplex DNA binds to the fingers domain, and its orientation relative to upstream DNA in the initiation complex implies an unwinding that could facilitate formation of the open promoter complex.

Original languageEnglish (US)
Title of host publicationStructural Insights into Gene Expression and Protein Synthesis
PublisherWorld Scientific Publishing Co.
Pages333-341
Number of pages9
ISBN (Electronic)9789811215865
ISBN (Print)9789811215858
StatePublished - Jan 1 2020
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Structural basis for the transition from initiation to elongation transcription in T7 RNA polymerase'. Together they form a unique fingerprint.

Cite this