Structural basis of broad ebolavirus neutralization by a human survivor antibody

Brandyn R. West; Anna Z. Wec; Crystal L. Moyer; Marnie L. Fusco; Philipp A. Ilinykh; Kai Huang; Ariel S. Wirchnianski; Rebekah M. James; Andrew S. Herbert; Sean Hui; Eileen Goodwin; Katie A. Howell; Shweta Kailasan; M. Javad Aman; Laura M. Walker; John M. Dye; Alexander Bukreyev; Kartik Chandran; Erica Ollmann Saphire

doi:10.1038/s41594-019-0191-4

Structural basis of broad ebolavirus neutralization by a human survivor antibody

Brandyn R. West, Anna Z. Wec, Crystal L. Moyer, Marnie L. Fusco, Philipp A. Ilinykh, Kai Huang, Ariel S. Wirchnianski, Rebekah M. James, Andrew S. Herbert, Sean Hui, Eileen Goodwin, Katie A. Howell, Shweta Kailasan, M. Javad Aman, Laura M. Walker, John M. Dye, Alexander Bukreyev, Kartik Chandran, Erica Ollmann Saphire

Pathology

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27 Scopus citations

Abstract

The structural features that govern broad-spectrum activity of broadly neutralizing anti-ebolavirus antibodies (Abs) outside of the internal fusion loop epitope are currently unknown. Here we describe the structure of a broadly neutralizing human monoclonal Ab (mAb), ADI-15946, which was identified in a human survivor of the 2013–2016 outbreak. The crystal structure of ADI-15946 in complex with cleaved Ebola virus glycoprotein (EBOV GP_CL) reveals that binding of the mAb structurally mimics the conserved interaction between the EBOV GP core and its glycan cap β17–β18 loop to inhibit infection. Both endosomal proteolysis of EBOV GP and binding of mAb FVM09 displace this loop, thereby increasing exposure of ADI-15946’s conserved epitope and enhancing neutralization. Our work also mapped the paratope of ADI-15946, thereby explaining reduced activity against Sudan virus, which enabled rational, structure-guided engineering to enhance binding and neutralization of Sudan virus while retaining the parental activity against EBOV and Bundibugyo virus.

Original language	English (US)
Pages (from-to)	204-212
Number of pages	9
Journal	Nature Structural and Molecular Biology
Volume	26
Issue number	3
DOIs	https://doi.org/10.1038/s41594-019-0191-4
State	Published - Mar 1 2019

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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West, B. R., Wec, A. Z., Moyer, C. L., Fusco, M. L., Ilinykh, P. A., Huang, K., Wirchnianski, A. S., James, R. M., Herbert, A. S., Hui, S., Goodwin, E., Howell, K. A., Kailasan, S., Aman, M. J., Walker, L. M., Dye, J. M., Bukreyev, A., Chandran, K., & Saphire, E. O. (2019). Structural basis of broad ebolavirus neutralization by a human survivor antibody. Nature Structural and Molecular Biology, 26(3), 204-212. https://doi.org/10.1038/s41594-019-0191-4

West, BR, Wec, AZ, Moyer, CL, Fusco, ML, Ilinykh, PA, Huang, K, Wirchnianski, AS, James, RM, Herbert, AS, Hui, S, Goodwin, E, Howell, KA, Kailasan, S, Aman, MJ, Walker, LM, Dye, JM, Bukreyev, A, Chandran, K & Saphire, EO 2019, 'Structural basis of broad ebolavirus neutralization by a human survivor antibody', Nature Structural and Molecular Biology, vol. 26, no. 3, pp. 204-212. https://doi.org/10.1038/s41594-019-0191-4

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abstract = "The structural features that govern broad-spectrum activity of broadly neutralizing anti-ebolavirus antibodies (Abs) outside of the internal fusion loop epitope are currently unknown. Here we describe the structure of a broadly neutralizing human monoclonal Ab (mAb), ADI-15946, which was identified in a human survivor of the 2013–2016 outbreak. The crystal structure of ADI-15946 in complex with cleaved Ebola virus glycoprotein (EBOV GPCL) reveals that binding of the mAb structurally mimics the conserved interaction between the EBOV GP core and its glycan cap β17–β18 loop to inhibit infection. Both endosomal proteolysis of EBOV GP and binding of mAb FVM09 displace this loop, thereby increasing exposure of ADI-15946{\textquoteright}s conserved epitope and enhancing neutralization. Our work also mapped the paratope of ADI-15946, thereby explaining reduced activity against Sudan virus, which enabled rational, structure-guided engineering to enhance binding and neutralization of Sudan virus while retaining the parental activity against EBOV and Bundibugyo virus.",

author = "West, {Brandyn R.} and Wec, {Anna Z.} and Moyer, {Crystal L.} and Fusco, {Marnie L.} and Ilinykh, {Philipp A.} and Kai Huang and Wirchnianski, {Ariel S.} and James, {Rebekah M.} and Herbert, {Andrew S.} and Sean Hui and Eileen Goodwin and Howell, {Katie A.} and Shweta Kailasan and Aman, {M. Javad} and Walker, {Laura M.} and Dye, {John M.} and Alexander Bukreyev and Kartik Chandran and Saphire, {Erica Ollmann}",

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Structural basis of broad ebolavirus neutralization by a human survivor antibody

Abstract

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