Structural changes of bacteriophage φ29 upon DNA packaging and release

Ye Xiang, Marc Morais, Anthony J. Battisti, Shelley Grimes, Paul J. Jardine, Dwight L. Anderson, Michael G. Rossmann

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Cryo-electron microscopy three-dimensional reconstructions have been made of mature and of emptied bacteriophage φ29 particles without making symmetry assumptions. Comparisons of these structures with each other and with the φ29 prohead indicate how conformational changes might initiate successive steps of assembly and infection. The 12 adsorption capable 'appendages' were found to have a structure homologous to the bacteriophage P22 tailspikes. Two of the appendages are extended radially outwards, away from the long axis of the virus, whereas the others are around and parallel to the phage axis. The appendage orientations are correlated with the symmetry-mismatched positions of the five-fold related head fibers, suggesting a mechanism for partial cell wall digestion upon rotation of the head about the tail when initiating infection. The narrow end of the head-tail connector is expanded in the mature virus. Gene product 3, bound to the 5′ ends of the genome, appears to be positioned within the expanded connector, which may potentiate the release of DNA-packaging machine components, creating a binding site for attachment of the tail.

Original languageEnglish (US)
Pages (from-to)5229-5239
Number of pages11
JournalEMBO Journal
Volume25
Issue number21
DOIs
StatePublished - Nov 1 2006
Externally publishedYes

Fingerprint

DNA Packaging
Bacteriophages
Packaging
Head
Viruses
DNA
Genes
Packaging machines
Bacteriophage P22
Cryoelectron Microscopy
Machine components
Infection
Cell Wall
Electron microscopy
Adsorption
Digestion
Binding Sites
Cells
Genome
Fibers

Keywords

  • φ29 structure
  • Cryo-electron microscopy
  • DNA ejection
  • DNA packaging

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Xiang, Y., Morais, M., Battisti, A. J., Grimes, S., Jardine, P. J., Anderson, D. L., & Rossmann, M. G. (2006). Structural changes of bacteriophage φ29 upon DNA packaging and release. EMBO Journal, 25(21), 5229-5239. https://doi.org/10.1038/sj.emboj.7601386

Structural changes of bacteriophage φ29 upon DNA packaging and release. / Xiang, Ye; Morais, Marc; Battisti, Anthony J.; Grimes, Shelley; Jardine, Paul J.; Anderson, Dwight L.; Rossmann, Michael G.

In: EMBO Journal, Vol. 25, No. 21, 01.11.2006, p. 5229-5239.

Research output: Contribution to journalArticle

Xiang, Y, Morais, M, Battisti, AJ, Grimes, S, Jardine, PJ, Anderson, DL & Rossmann, MG 2006, 'Structural changes of bacteriophage φ29 upon DNA packaging and release', EMBO Journal, vol. 25, no. 21, pp. 5229-5239. https://doi.org/10.1038/sj.emboj.7601386
Xiang Y, Morais M, Battisti AJ, Grimes S, Jardine PJ, Anderson DL et al. Structural changes of bacteriophage φ29 upon DNA packaging and release. EMBO Journal. 2006 Nov 1;25(21):5229-5239. https://doi.org/10.1038/sj.emboj.7601386
Xiang, Ye ; Morais, Marc ; Battisti, Anthony J. ; Grimes, Shelley ; Jardine, Paul J. ; Anderson, Dwight L. ; Rossmann, Michael G. / Structural changes of bacteriophage φ29 upon DNA packaging and release. In: EMBO Journal. 2006 ; Vol. 25, No. 21. pp. 5229-5239.
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