Structural characterization of a monomeric chemokine: Monocyte chemoattractant protein-3

Kim Key-Sun; Krishnakumar Rajarathnam; Ian Clark-Lewis; Brian D. Sykes

doi:10.1016/0014-5793(96)01024-1

Structural characterization of a monomeric chemokine: Monocyte chemoattractant protein-3

Kim Key-Sun, Krishnakumar Rajarathnam, Ian Clark-Lewis, Brian D. Sykes

Research output: Contribution to journal › Article › peer-review

75 Scopus citations

Abstract

¹H-NMR spectroscopy and analytical ultracentrifugation studies reveal that monocyte chemoattractant protein-3 (MCP-3) is a monomer. NMR solution structure shows that MCP-3 adopts an α/β fold similar to what is observed in structures of other known chemokines. However, MCP-3 is unique in that it does not show a propensity to form dimers. The closely related chemokines MCP-1 and MCP-3 show a monomer-dimer equilibrium in sedimentation equilibrium studies (~ 0.2-2 mg/ml). As these proteins are present at nanomolar concentrations in vivo, the results suggest that they are monomeric at functional concentrations and that the monomer is the functionally significant form of MCP-1, MCP-2 and MCP-3.

Original language	English (US)
Pages (from-to)	277-282
Number of pages	6
Journal	FEBS Letters
Volume	395
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-5793(96)01024-1
State	Published - Oct 21 1996
Externally published	Yes

Keywords

Monocyte chemotactic protein
Monomer-dimer
NMR
Solution structure

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(96)01024-1

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title = "Structural characterization of a monomeric chemokine: Monocyte chemoattractant protein-3",

abstract = "1H-NMR spectroscopy and analytical ultracentrifugation studies reveal that monocyte chemoattractant protein-3 (MCP-3) is a monomer. NMR solution structure shows that MCP-3 adopts an α/β fold similar to what is observed in structures of other known chemokines. However, MCP-3 is unique in that it does not show a propensity to form dimers. The closely related chemokines MCP-1 and MCP-3 show a monomer-dimer equilibrium in sedimentation equilibrium studies (~ 0.2-2 mg/ml). As these proteins are present at nanomolar concentrations in vivo, the results suggest that they are monomeric at functional concentrations and that the monomer is the functionally significant form of MCP-1, MCP-2 and MCP-3.",

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T1 - Structural characterization of a monomeric chemokine

T2 - Monocyte chemoattractant protein-3

AU - Key-Sun, Kim

AU - Rajarathnam, Krishnakumar

AU - Clark-Lewis, Ian

AU - Sykes, Brian D.

PY - 1996/10/21

Y1 - 1996/10/21

N2 - 1H-NMR spectroscopy and analytical ultracentrifugation studies reveal that monocyte chemoattractant protein-3 (MCP-3) is a monomer. NMR solution structure shows that MCP-3 adopts an α/β fold similar to what is observed in structures of other known chemokines. However, MCP-3 is unique in that it does not show a propensity to form dimers. The closely related chemokines MCP-1 and MCP-3 show a monomer-dimer equilibrium in sedimentation equilibrium studies (~ 0.2-2 mg/ml). As these proteins are present at nanomolar concentrations in vivo, the results suggest that they are monomeric at functional concentrations and that the monomer is the functionally significant form of MCP-1, MCP-2 and MCP-3.

AB - 1H-NMR spectroscopy and analytical ultracentrifugation studies reveal that monocyte chemoattractant protein-3 (MCP-3) is a monomer. NMR solution structure shows that MCP-3 adopts an α/β fold similar to what is observed in structures of other known chemokines. However, MCP-3 is unique in that it does not show a propensity to form dimers. The closely related chemokines MCP-1 and MCP-3 show a monomer-dimer equilibrium in sedimentation equilibrium studies (~ 0.2-2 mg/ml). As these proteins are present at nanomolar concentrations in vivo, the results suggest that they are monomeric at functional concentrations and that the monomer is the functionally significant form of MCP-1, MCP-2 and MCP-3.

KW - Monocyte chemotactic protein

KW - Monomer-dimer

KW - NMR

KW - Solution structure

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Structural characterization of a monomeric chemokine: Monocyte chemoattractant protein-3

Abstract

Keywords

ASJC Scopus subject areas

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