Structural characterization of the GM1 ganglioside by infrared multiphoton dissociation, electron capture dissociation, and electron detachment dissociation electrospray ionization FT-ICR MS/MS

Melinda A. McFarland, Alan G. Marshall, Christopher L. Hendrickson, Carol L. Nilsson, Pam Fredman, Jan Eric Månsson

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Gangliosides play important biological roles and structural characterization of both the carbohydrate and the lipid moieties is important. The FT-ICR MS/MS techniques of electron capture dissociation (ECD), electron detachment dissociation (EDD), and infrared multiphoton dissociation (IRMPD) provide extensive fragmentation of the protonated and deprotonated GM1 ganglioside. ECD provides extensive structural information, including identification of both halves of the ceramide and cleavage of the acetyl moiety of the N-acetylated sugars. IRMPD provides similar glycan fragmentation but no cleavage of the acetyl moiety. Cleavage between the fatty acid and the long-chain base of the ceramide moiety is seen in negative-ion IRMPD but not in positive-ion IRMPD of GM1. Furthermore, this extent of fragmentation requires a range of laser powers, whereas all information is available from a single ECD experiment. However, stepwise fragmentation by IRMPD may be used to map the relative labilities for a series of cleavages. EDD provides the alternative of electron-induced fragmentation for negative ions with extensive fragmentation, but suffers from low efficiency as well as complication of data analysis by frequent loss of hydrogen atoms. We also show that analysis of MS/MS data for glycolipids is greatly simplified by classification of product ion masses to specific regions of the ganglioside based solely on mass defect graphical analysis.

Original languageEnglish (US)
Pages (from-to)752-762
Number of pages11
JournalJournal of the American Society for Mass Spectrometry
Volume16
Issue number5
DOIs
StatePublished - May 2005
Externally publishedYes

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G(M1) Ganglioside
Electrospray ionization
Electrons
Infrared radiation
Ions
Gangliosides
Ceramides
Glycolipids
Negative ions
Polysaccharides
Hydrogen
Lasers
Fatty Acids
Carbohydrates
Lipids
Sugars
Positive ions

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

Cite this

Structural characterization of the GM1 ganglioside by infrared multiphoton dissociation, electron capture dissociation, and electron detachment dissociation electrospray ionization FT-ICR MS/MS. / McFarland, Melinda A.; Marshall, Alan G.; Hendrickson, Christopher L.; Nilsson, Carol L.; Fredman, Pam; Månsson, Jan Eric.

In: Journal of the American Society for Mass Spectrometry, Vol. 16, No. 5, 05.2005, p. 752-762.

Research output: Contribution to journalArticle

McFarland, Melinda A. ; Marshall, Alan G. ; Hendrickson, Christopher L. ; Nilsson, Carol L. ; Fredman, Pam ; Månsson, Jan Eric. / Structural characterization of the GM1 ganglioside by infrared multiphoton dissociation, electron capture dissociation, and electron detachment dissociation electrospray ionization FT-ICR MS/MS. In: Journal of the American Society for Mass Spectrometry. 2005 ; Vol. 16, No. 5. pp. 752-762.
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