Structural determinants of metal specificity in the zinc transport protein ZnuA from Synechocystis 6803

Soojay Banerjee, Baoxian Wei, Maitrayee Bhattacharyya-Pakrasi, Himadri B. Pakrasi, Thomas Smith

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

A number of bacterial metal transporters belong to the cluster 9 family of ABC transporters. The residues in the periplasmic domain thought to be involved in metal binding seem highly conserved and yet the transporters have varying metal specificity. To solve this seeming paradox and ascertain how metal specificity is exacted, the structure of ZnuA, the periplasmic domain of a zinc transporter from Synechocystis 6803, has been determined to a resolution of 1.9Å. In previously determined structures of homologous proteins, four residues chelate the bound metal. From sequence alignments of the cluster 9 metal transporters, the fourth residue in this metal-binding site, an aspartate, is also present in the appropriate position in the ZnuA sequence. However, this result is misleading, since our structural data indicate that zinc binds via only three histidine residues and the aspartate is replaced by a large hydrophobic cavity. We propose that ZnuA binds zinc over manganese by providing only three ligating residues. ZnuA has a highly charged and mobile loop that protrudes from the protein in the vicinity of the metal-binding site. Similar loops are found in other types of zinc transporters but not manganese transporters. Therefore, we propose that the function of this domain is to act as a zinc chaperone to facilitate acquisition. Therefore, while Mn2+ transporters can bind Zn2+ in vitro they may not be able to acquire it in vivo without this structure because of the low concentration of free Zn2+.

Original languageEnglish (US)
Pages (from-to)1061-1069
Number of pages9
JournalJournal of Molecular Biology
Volume333
Issue number5
DOIs
StatePublished - Nov 7 2003
Externally publishedYes

Fingerprint

Synechocystis
Zinc
Carrier Proteins
Metals
Manganese
Aspartic Acid
Binding Sites
ATP-Binding Cassette Transporters
Sequence Alignment
Histidine
Proteins

Keywords

  • Crystallography
  • Metal
  • Specificity
  • Transport
  • Zinc

ASJC Scopus subject areas

  • Virology

Cite this

Structural determinants of metal specificity in the zinc transport protein ZnuA from Synechocystis 6803. / Banerjee, Soojay; Wei, Baoxian; Bhattacharyya-Pakrasi, Maitrayee; Pakrasi, Himadri B.; Smith, Thomas.

In: Journal of Molecular Biology, Vol. 333, No. 5, 07.11.2003, p. 1061-1069.

Research output: Contribution to journalArticle

Banerjee, Soojay ; Wei, Baoxian ; Bhattacharyya-Pakrasi, Maitrayee ; Pakrasi, Himadri B. ; Smith, Thomas. / Structural determinants of metal specificity in the zinc transport protein ZnuA from Synechocystis 6803. In: Journal of Molecular Biology. 2003 ; Vol. 333, No. 5. pp. 1061-1069.
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