Structural determinants of spectral tuning in retinal proteins - Bacteriorhodopsin vs sensory rhodopsin II

Shigehiko Hayashi, Emad Tajkhorshid, Eva Pebay-Peyroula, Antoine Royant, Ehud M. Landau, Javier Navarro, Klaus Schulten

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

The mechanism of spectral tuning in the rhodopsin family of proteins, that act as light-driven proton (ion) pumps and light detectors, has been investigated by a combined ab initio quantum mechanical/molecular mechanical technique. Calculations are performed on two members of the family, bacteriorhodopsin (bR) and sensory rhodopsin II (sRII), for which crystal structures of high resolution are available, to explore the physical mechanisms of spectral tuning. Despite a high degree of similarity in the three-dimensional structure, electrostatic environments in bR and sRII differ sufficiently to shift absorption maxima of their common chromophore, a retinal bound to a lysine via a protonated Schiff base, from 568 nm in bR to 497 nm in sRII. This spectral shift, involving the electronical ground state (S0) and first excited state (S1) of retinal, is predicted correctly within 10 nm. The spectral shift can be attributed predominantly to a change in polarization of the S1 state, and is induced predominantly by a shift of the G helix that renders the distance between the Schiff base nitrogen of retinal and the Asp201 counterion shorter in sRII than in bR. A second, weakly allowed excited state, S2, is predicted to lie energetically close to S1, at 474 nm. Its energetic proximity to the S1 state suggests strong vibronic coupling and explains a shoulder observed at 457 nm in the sRII spectrum.

Original languageEnglish (US)
Pages (from-to)10124-10131
Number of pages8
JournalJournal of Physical Chemistry B
Volume105
Issue number41
DOIs
StatePublished - Oct 18 2001

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Sensory Rhodopsins
Bacteriorhodopsins
Excited states
determinants
Tuning
tuning
proteins
Proteins
shift
Chromophores
Photodetectors
Schiff Bases
Ground state
imines
Electrostatics
Protons
Crystal structure
Pumps
ion pumps
Polarization

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

Hayashi, S., Tajkhorshid, E., Pebay-Peyroula, E., Royant, A., Landau, E. M., Navarro, J., & Schulten, K. (2001). Structural determinants of spectral tuning in retinal proteins - Bacteriorhodopsin vs sensory rhodopsin II. Journal of Physical Chemistry B, 105(41), 10124-10131. https://doi.org/10.1021/jp011362b

Structural determinants of spectral tuning in retinal proteins - Bacteriorhodopsin vs sensory rhodopsin II. / Hayashi, Shigehiko; Tajkhorshid, Emad; Pebay-Peyroula, Eva; Royant, Antoine; Landau, Ehud M.; Navarro, Javier; Schulten, Klaus.

In: Journal of Physical Chemistry B, Vol. 105, No. 41, 18.10.2001, p. 10124-10131.

Research output: Contribution to journalArticle

Hayashi, S, Tajkhorshid, E, Pebay-Peyroula, E, Royant, A, Landau, EM, Navarro, J & Schulten, K 2001, 'Structural determinants of spectral tuning in retinal proteins - Bacteriorhodopsin vs sensory rhodopsin II', Journal of Physical Chemistry B, vol. 105, no. 41, pp. 10124-10131. https://doi.org/10.1021/jp011362b
Hayashi, Shigehiko ; Tajkhorshid, Emad ; Pebay-Peyroula, Eva ; Royant, Antoine ; Landau, Ehud M. ; Navarro, Javier ; Schulten, Klaus. / Structural determinants of spectral tuning in retinal proteins - Bacteriorhodopsin vs sensory rhodopsin II. In: Journal of Physical Chemistry B. 2001 ; Vol. 105, No. 41. pp. 10124-10131.
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