Structural proteins of La Crosse virus

J. F. Obijeski, D. H L Bishop, F. A. Murphy, E. L. Palmer

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Preparations of La Crosse virus, a member of the California encephalitis group of bunyaviruses, were found to possess three major virion proteins. Two of the proteins were glycosylated (G1 and G2) and were located on the surface of the virus particles. These two glycoproteins were present in equimolar amounts and possessed apparent molecular weights of 120x103 and 34x103. Virion nucleocapsids, isolated by a nonionic detergent and salt treatment, contained another major protein, N (molecular weight=23x103). A large, but minor, protein species L (molecular weight=180x103) was also found in virus preparations. The approximate number of protein molecules per virion has been determined. Electron microscopy of purified La Crosse virus indicated that the virus particle (mean diameter, 91 nm) is enveloped and possesses irregular surface projections (length, 10 nm).

Original languageEnglish (US)
Pages (from-to)985-997
Number of pages13
JournalJournal of Virology
Volume19
Issue number3
StatePublished - 1976

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La Crosse virus
California encephalitis virus
structural proteins
virion
Virion
Molecular Weight
molecular weight
Proteins
proteins
California Encephalitis
Orthobunyavirus
Nucleocapsid
nucleocapsid
encephalitis
detergents
Detergents
glycoproteins
electron microscopy
Electron Microscopy
Glycoproteins

ASJC Scopus subject areas

  • Immunology

Cite this

Obijeski, J. F., Bishop, D. H. L., Murphy, F. A., & Palmer, E. L. (1976). Structural proteins of La Crosse virus. Journal of Virology, 19(3), 985-997.

Structural proteins of La Crosse virus. / Obijeski, J. F.; Bishop, D. H L; Murphy, F. A.; Palmer, E. L.

In: Journal of Virology, Vol. 19, No. 3, 1976, p. 985-997.

Research output: Contribution to journalArticle

Obijeski, JF, Bishop, DHL, Murphy, FA & Palmer, EL 1976, 'Structural proteins of La Crosse virus', Journal of Virology, vol. 19, no. 3, pp. 985-997.
Obijeski JF, Bishop DHL, Murphy FA, Palmer EL. Structural proteins of La Crosse virus. Journal of Virology. 1976;19(3):985-997.
Obijeski, J. F. ; Bishop, D. H L ; Murphy, F. A. ; Palmer, E. L. / Structural proteins of La Crosse virus. In: Journal of Virology. 1976 ; Vol. 19, No. 3. pp. 985-997.
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