Structure-activity determinants in antifungal plant defensins msdef1 and mtdef4 with different modes of action against fusarium graminearum

Uma Shankar Sagaram, Raghoottama Pandurangi, Jagdeep Kaur, Thomas Smith, Dilip M. Shah

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Plant defensins are small cysteine-rich antimicrobial proteins. Their three-dimensional structures are similar in that they consist of an α-helix and three anti-parallel β-strands stabilized by four disulfide bonds. Plant defensins MsDef1 and MtDef4 are potent inhibitors of the growth of several filamentous fungi including Fusarium graminearum. However, they differ markedly in their antifungal properties as well as modes of antifungal action. MsDef1 induces prolific hyperbranching of fungal hyphae, whereas MtDef4 does not. Both defensins contain a highly conserved γ-core motif (GXCX3-9C), a hallmark signature present in the disulfide-stabilized antimicrobial peptides, composed of β2 and β3 strands and the interposed loop. The γ-core motifs of these two defensins differ significantly in their primary amino acid sequences and in their net charge. In this study, we have found that the major determinants of the antifungal activity and morphogenicity of these defensins reside in their γ-core motifs. The MsDef1-γ4 variant in which the γ-core motif of MsDef1 was replaced by that of MtDef4 was almost as potent as MtDef4 and also failed to induce hyperbranching of fungal hyphae. Importantly, the γ-core motif of MtDef4 alone was capable of inhibiting fungal growth, but that of MsDef1 was not. The analysis of synthetic γ-core variants of MtDef4 indicated that the cationic and hydrophobic amino acids were important for antifungal activity. Both MsDef1 and MtDef4 induced plasma membrane permeabilization; however, kinetic studies revealed that MtDef4 was more efficient in permeabilizing fungal plasma membrane than MsDef1. Furthermore, the in vitro antifungal activity of MsDef1, MsDef1-γ4, MtDef4 and peptides derived from the γ-core motif of each defensin was not solely dependent on their ability to permeabilize the fungal plasma membrane. The data reported here indicate that the γ-core motif defines the unique antifungal properties of each defensin and may facilitate de novo design of more potent antifungal peptides.

Original languageEnglish (US)
Article numbere18550
JournalPLoS One
Volume6
Issue number4
DOIs
StatePublished - 2011
Externally publishedYes

Fingerprint

Defensins
Fusarium graminearum
Fusarium
antifungal properties
mechanism of action
plasma membrane
antimicrobial peptides
hyphae
antimicrobial proteins
Cell membranes
disulfide bonds
Hyphae
sulfides
Cell Membrane
cysteine
microbial growth
Disulfides
Peptides
amino acid sequences
peptides

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Structure-activity determinants in antifungal plant defensins msdef1 and mtdef4 with different modes of action against fusarium graminearum. / Sagaram, Uma Shankar; Pandurangi, Raghoottama; Kaur, Jagdeep; Smith, Thomas; Shah, Dilip M.

In: PLoS One, Vol. 6, No. 4, e18550, 2011.

Research output: Contribution to journalArticle

Sagaram, Uma Shankar ; Pandurangi, Raghoottama ; Kaur, Jagdeep ; Smith, Thomas ; Shah, Dilip M. / Structure-activity determinants in antifungal plant defensins msdef1 and mtdef4 with different modes of action against fusarium graminearum. In: PLoS One. 2011 ; Vol. 6, No. 4.
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