Structure and location of gene product 8 in the bacteriophage T4 baseplate

Petr Leiman, Mikhail M. Shneider, Victor A. Kostyuchenko, Paul R. Chipman, Vadim V. Mesyanzhinov, Michael G. Rossmann

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Many bacteriophages, such as T4, T7, RB49, and φ29, have complex, sometimes multilayered, tails that facilitate an almost 100% success rate for the viral particles to infect host cells. In bacteriophage T4, there is a baseplate, which is a multiprotein assembly, at the distal end of the contractile tail. The baseplate communicates to the tail that the phage fibers have attached to the host cell, thereby initiating the infection process. Gene product 8 (gp8), whose amino acid sequence consists of 334 residues, is one of at least 16 different structural proteins that constitute the T4 baseplate and is the sixth baseplate protein whose structure has been determined. A 2.0Å resolution X-ray structure of gp8 shows that the two-domain protein forms a dimer, in which each monomer consists of a three-layered β-sandwich with two loops, each containing an α-helix at the opposite sides of the sandwich. The crystals of gp8 were produced in the presence of concentrated chloride and bromide ions, resulting in at least 11 halide-binding sites per monomer. Five halide sites, situated at the N termini of α-helices, have a protein environment observed in other halide-containing protein crystal structures. The computer programs EMfit and SITUS were used to determine the positions of six gp8 dimers within the 12Å resolution cryo-electron microscopy image reconstruction of the baseplate-tail tube complex. The gp8 dimers were found to be located in the upper part of the baseplate outer rim. About 20% of the gp8 surface is involved in contacts with other baseplate proteins, presumed to be gp6, gp7, and gp10. With the structure determination of gp8, a total of 53% of the volume of the baseplate has now been interpreted in terms of its atomic structure.

Original languageEnglish (US)
Pages (from-to)821-833
Number of pages13
JournalJournal of Molecular Biology
Volume328
Issue number4
DOIs
StatePublished - May 9 2003
Externally publishedYes

Fingerprint

Bacteriophage T4
Genes
Proteins
Bacteriophages
Cryoelectron Microscopy
Gene Order
Computer-Assisted Image Processing
Bromides
Virion
Chlorides
Amino Acid Sequence
Software
Binding Sites
X-Rays
Ions
Infection

Keywords

  • Bacteriophage
  • Baseplate
  • Gene product 8
  • Structure
  • T4

ASJC Scopus subject areas

  • Virology

Cite this

Leiman, P., Shneider, M. M., Kostyuchenko, V. A., Chipman, P. R., Mesyanzhinov, V. V., & Rossmann, M. G. (2003). Structure and location of gene product 8 in the bacteriophage T4 baseplate. Journal of Molecular Biology, 328(4), 821-833. https://doi.org/10.1016/S0022-2836(03)00366-8

Structure and location of gene product 8 in the bacteriophage T4 baseplate. / Leiman, Petr; Shneider, Mikhail M.; Kostyuchenko, Victor A.; Chipman, Paul R.; Mesyanzhinov, Vadim V.; Rossmann, Michael G.

In: Journal of Molecular Biology, Vol. 328, No. 4, 09.05.2003, p. 821-833.

Research output: Contribution to journalArticle

Leiman, P, Shneider, MM, Kostyuchenko, VA, Chipman, PR, Mesyanzhinov, VV & Rossmann, MG 2003, 'Structure and location of gene product 8 in the bacteriophage T4 baseplate', Journal of Molecular Biology, vol. 328, no. 4, pp. 821-833. https://doi.org/10.1016/S0022-2836(03)00366-8
Leiman P, Shneider MM, Kostyuchenko VA, Chipman PR, Mesyanzhinov VV, Rossmann MG. Structure and location of gene product 8 in the bacteriophage T4 baseplate. Journal of Molecular Biology. 2003 May 9;328(4):821-833. https://doi.org/10.1016/S0022-2836(03)00366-8
Leiman, Petr ; Shneider, Mikhail M. ; Kostyuchenko, Victor A. ; Chipman, Paul R. ; Mesyanzhinov, Vadim V. ; Rossmann, Michael G. / Structure and location of gene product 8 in the bacteriophage T4 baseplate. In: Journal of Molecular Biology. 2003 ; Vol. 328, No. 4. pp. 821-833.
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