Structure of arp2/3 complex in its activated state and in actin filament branch junctions

N. Volkmann, K. J. Amann, S. Stoilova-McPhie, C. Egile, D. C. Winter, L. Hazelwood, J. E. Heuser, R. Li, T. D. Pollard, D. Hanein

Research output: Contribution to journalArticle

188 Citations (Scopus)

Abstract

The seven-subunit Arp2/3 complex choreographs the formation of branched actin networks at the leading edge of migrating cells. When activated by Wiskott-Aldrich Syndrome protein (WASp), the Arp2/3 complex initiates actin filament branches from the sides of existing filaments. Electron cryomicroscopy and three-dimensional reconstruction of Acanthamoeba castellanii and Saccharomyces cerevisiae Arp2/3 complexes bound to the WASp carboxy-terminal domain reveal asymmetric, oblate ellipsoids. Image analysis of actin branches indicates that the complex binds the side of the mother filament, and Arp2 and Arp3 (for actin-related protein) are the first two subunits of the daughter filament. Comparison to the actin-free, WASp-activated complexes suggests that branch initiation involves large-scale structural rearrangements within Arp2/3.

Original languageEnglish (US)
Pages (from-to)2456-2459
Number of pages4
JournalScience
Volume293
Issue number5539
DOIs
StatePublished - Sep 28 2001
Externally publishedYes

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Actin-Related Protein 2-3 Complex
Wiskott-Aldrich Syndrome Protein
Actin Cytoskeleton
Actins
Acanthamoeba castellanii
Cryoelectron Microscopy
Saccharomyces cerevisiae
Proteins

ASJC Scopus subject areas

  • General

Cite this

Volkmann, N., Amann, K. J., Stoilova-McPhie, S., Egile, C., Winter, D. C., Hazelwood, L., ... Hanein, D. (2001). Structure of arp2/3 complex in its activated state and in actin filament branch junctions. Science, 293(5539), 2456-2459. https://doi.org/10.1126/science.1063025

Structure of arp2/3 complex in its activated state and in actin filament branch junctions. / Volkmann, N.; Amann, K. J.; Stoilova-McPhie, S.; Egile, C.; Winter, D. C.; Hazelwood, L.; Heuser, J. E.; Li, R.; Pollard, T. D.; Hanein, D.

In: Science, Vol. 293, No. 5539, 28.09.2001, p. 2456-2459.

Research output: Contribution to journalArticle

Volkmann, N, Amann, KJ, Stoilova-McPhie, S, Egile, C, Winter, DC, Hazelwood, L, Heuser, JE, Li, R, Pollard, TD & Hanein, D 2001, 'Structure of arp2/3 complex in its activated state and in actin filament branch junctions', Science, vol. 293, no. 5539, pp. 2456-2459. https://doi.org/10.1126/science.1063025
Volkmann N, Amann KJ, Stoilova-McPhie S, Egile C, Winter DC, Hazelwood L et al. Structure of arp2/3 complex in its activated state and in actin filament branch junctions. Science. 2001 Sep 28;293(5539):2456-2459. https://doi.org/10.1126/science.1063025
Volkmann, N. ; Amann, K. J. ; Stoilova-McPhie, S. ; Egile, C. ; Winter, D. C. ; Hazelwood, L. ; Heuser, J. E. ; Li, R. ; Pollard, T. D. ; Hanein, D. / Structure of arp2/3 complex in its activated state and in actin filament branch junctions. In: Science. 2001 ; Vol. 293, No. 5539. pp. 2456-2459.
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