TY - JOUR
T1 - Structure of Cowpea mottle virus
T2 - A consensus in the genus Carmovirus
AU - Ke, Jiyuan
AU - Schmidt, Timothy
AU - Chase, Elaine
AU - Bozarth, Robert F.
AU - Smith, Thomas J.
N1 - Funding Information:
The authors would like to thank Dr. S.C. Harrison for providing the coordinates of TBSV and TCV, and to Dr. D.I. Stuart for providing the coordinates of CarMV. This work is supported by an NIH grant to T.J.S. (GM10704). Figs. 4 and 5 were produced using the program MolViewX ( http://www.danforthcenter.org/smith : Smith, 1995 ).
PY - 2004/4/10
Y1 - 2004/4/10
N2 - Cowpea mottle virus (CPMoV) is a T = 3 virus that belongs to Carmovirus genus of the Tombusviridae family. Here, we report the crystal structure of CPMoV determined to a resolution of 7.0 Å. The structures and sequences of three Carmoviruses, CPMoV, Turnip crinkle virus (TCV), and Carnation mottle virus (CarMV) have been compared to TBSV from the Tombusvirus genus. CPMoV, TCV, and CarMV all have a deletion in βC strand in the S domain relative to TBSV that may be distinctive to the genus. Although CPMoV has an elongated C-terminus like TBSV, it does not interact with the icosahedrally related P domain as observed in TBSV. In CPMoV, the termini of A and B interact with the icosahedrally related shell domains of A and C, respectively, to form a chain of interactions around the 5-fold axes. The C subunit terminus does not, however, interact with the B subunit because of quasi-equivalent differences in the P domain orientations.
AB - Cowpea mottle virus (CPMoV) is a T = 3 virus that belongs to Carmovirus genus of the Tombusviridae family. Here, we report the crystal structure of CPMoV determined to a resolution of 7.0 Å. The structures and sequences of three Carmoviruses, CPMoV, Turnip crinkle virus (TCV), and Carnation mottle virus (CarMV) have been compared to TBSV from the Tombusvirus genus. CPMoV, TCV, and CarMV all have a deletion in βC strand in the S domain relative to TBSV that may be distinctive to the genus. Although CPMoV has an elongated C-terminus like TBSV, it does not interact with the icosahedrally related P domain as observed in TBSV. In CPMoV, the termini of A and B interact with the icosahedrally related shell domains of A and C, respectively, to form a chain of interactions around the 5-fold axes. The C subunit terminus does not, however, interact with the B subunit because of quasi-equivalent differences in the P domain orientations.
KW - Carmoviruses
KW - Crystallography
KW - Structure
KW - Tombusviridae
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U2 - 10.1016/j.virol.2004.01.018
DO - 10.1016/j.virol.2004.01.018
M3 - Article
C2 - 15051394
AN - SCOPUS:1642487718
SN - 0042-6822
VL - 321
SP - 349
EP - 358
JO - Virology
JF - Virology
IS - 2
ER -