Structure of isolated nucleocapsids from Venezuelan equine encephalitis virus and implications for assembly and disassembly of enveloped virus

Angel Paredes, Kathy Alwell-Warda, Scott Weaver, Wah Chiu, Stanley Watowich

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Venezuelan equine encephalitis virus (VEEV) is an important human and equine pathogen in the Americas, with widespread reoccurring epidemics extending from South America to the southern United States. Most troubling, VEEV has been made into a weapon by several countries and is currently restricted by the Centers for Disease Control and Prevention as a potential biological warfare and terrorism agent. To facilitate the development of antiviral compounds, the structure of the nucleocapsid isolated from VEEV has been determined by electron cryomicroscopy and image reconstruction and represents the first three-dimensional structure of a nucleocapsid isolated from a single-stranded enveloped RNA virus. The isolated VEEV nucleocapsid undergoes significant reorganization relative to its structure within VEEV. However, the isolated nucleocapsid clearly exhibits T=4 icosahedral symmetry, and its characteristic nucleocapsid hexons and pentons are preserved. The diameter of the isolated nucleocapsid is ∼11.5% larger than that of the nucleocapsid within VEEV, with radial expansion being greatest near the hexons. Significantly, this is the first direct structural evidence showing that a simple enveloped virus undergoes large conformational changes during maturation, suggesting that the lipid bilayer and the transmembrane proteins of simple enveloped viruses provide the energy necessary to reorganize the nucleocapsid during maturation.

Original languageEnglish (US)
Pages (from-to)659-664
Number of pages6
JournalJournal of Virology
Volume77
Issue number1
DOIs
StatePublished - Jan 2003

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Venezuelan Equine Encephalitis Viruses
Venezuelan equine encephalitis virus
Virus Assembly
Nucleocapsid
nucleocapsid
Viruses
viruses
Biological Warfare Agents
terrorism
Bioterrorism
Cryoelectron Microscopy
Centers for Disease Control and Prevention
lipid bilayers
transmembrane proteins
Weapons
Computer-Assisted Image Processing
South America
RNA Viruses
Lipid Bilayers
Centers for Disease Control and Prevention (U.S.)

ASJC Scopus subject areas

  • Immunology

Cite this

Structure of isolated nucleocapsids from Venezuelan equine encephalitis virus and implications for assembly and disassembly of enveloped virus. / Paredes, Angel; Alwell-Warda, Kathy; Weaver, Scott; Chiu, Wah; Watowich, Stanley.

In: Journal of Virology, Vol. 77, No. 1, 01.2003, p. 659-664.

Research output: Contribution to journalArticle

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