Structure of membrane-bound human factor Va

Svetla Stoylova, Kenneth G. Mann, Alain Brisson

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Coagulation factor Va is an essential cofactor which combines with the serine protease factor Xa on a phospholipid surface to form the prothrombinase complex. In the present study, the structure of factor Va interacting with lipid surfaces containing phosphatidylserine was studied by electron microscopy. Two-dimensional crystals of factor Va were obtained on planar lipid films under quasi-physiological conditions. The two-dimensional projected structure of factor Va was calculated at a resolution of 2 nm, revealing dimers of factor Va arranged on the surface lattice with the symmetry of the plane group p2. Average unit cell dimensions are a = 14.4 nm, b = 8.8 nm, γ = 107°. Each factor Va molecule presents two distinct domains of protein density consisting of one small domain, of 3 nm in diameter, connected to a larger domain of about 6 nm × 4.5 nm. The projected structure of factor Va covers an area equivalent to about fifty phospholipid molecules. In addition, edge-on views of factor Va molecules bound to liposomes reveal a globular structure connected through a thin stem to the liposome surface. A three-dimensional model of membrane-bound factor Va is proposed.

Original languageEnglish (US)
Pages (from-to)330-334
Number of pages5
JournalFEBS Letters
Volume351
Issue number3
DOIs
StatePublished - Sep 12 1994
Externally publishedYes

Fingerprint

Factor Va
Human engineering
Membranes
Liposomes
Molecules
Phospholipids
Lipids
Factor Xa
Phosphatidylserines
Serine Proteases
Dimers
Electron microscopy
Electron Microscopy

Keywords

  • Electron microscopy
  • Factor Va
  • Liposome
  • Phosphatidylserine
  • Two-dimensional crystal

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Stoylova, S., Mann, K. G., & Brisson, A. (1994). Structure of membrane-bound human factor Va. FEBS Letters, 351(3), 330-334. https://doi.org/10.1016/0014-5793(94)00881-7

Structure of membrane-bound human factor Va. / Stoylova, Svetla; Mann, Kenneth G.; Brisson, Alain.

In: FEBS Letters, Vol. 351, No. 3, 12.09.1994, p. 330-334.

Research output: Contribution to journalArticle

Stoylova, S, Mann, KG & Brisson, A 1994, 'Structure of membrane-bound human factor Va', FEBS Letters, vol. 351, no. 3, pp. 330-334. https://doi.org/10.1016/0014-5793(94)00881-7
Stoylova, Svetla ; Mann, Kenneth G. ; Brisson, Alain. / Structure of membrane-bound human factor Va. In: FEBS Letters. 1994 ; Vol. 351, No. 3. pp. 330-334.
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