Structure of the bacteriophage φ29 DNA packaging motor

Alan A. Simpson, Yizhi Tao, Petr Leiman, Mohammed O. Badasso, Yongning He, Paul J. Jardine, Norman H. Olson, Marc Morais, Shelley Grimes, Dwight L. Anderson, Timothy S. Baker, Michael G. Rossmann

Research output: Contribution to journalArticle

403 Citations (Scopus)

Abstract

Motors generating mechanical force, powered by the hydrolysis of ATP, translocate double-stranded DNA into preformed capsids (proheads) of bacterial viruses and certain animal viruses. Here we describe the motor that packages the double-stranded DNA of the Bacillus subtilis bacteriophage φ29 into a precursor capsid. We determined the structure of the head-tail connector-the central component of the φ29 DNA packaging motor-to 3.2Å resolution by means of X-ray crystallography. We then fitted the connector into the electron densities of the prohead and of the partially packaged prohead as determined using cryo-electron microscopy and image reconstruction analysis. Our results suggest that the prohead plus dodecameric connector, prohead RNA, viral ATPase and DNA comprise a rotary motor with the head-prohead RNA-ATPase complex acting as a stator, the DNA acting as a spindle, and the connector as a ball-race. The helical nature of the DNA converts the rotary action of the connector into translation of the DNA.

Original languageEnglish (US)
Pages (from-to)745-750
Number of pages6
JournalNature
Volume408
Issue number6813
DOIs
StatePublished - Dec 7 2000
Externally publishedYes

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DNA Packaging
Bacteriophages
DNA
Capsid
Adenosine Triphosphatases
Bacillus Phages
Head
RNA
Viruses
Cryoelectron Microscopy
Computer-Assisted Image Processing
X Ray Crystallography
Viral DNA
Bacillus subtilis
Hydrolysis
Adenosine Triphosphate
Electrons

ASJC Scopus subject areas

  • General

Cite this

Simpson, A. A., Tao, Y., Leiman, P., Badasso, M. O., He, Y., Jardine, P. J., ... Rossmann, M. G. (2000). Structure of the bacteriophage φ29 DNA packaging motor. Nature, 408(6813), 745-750. https://doi.org/10.1038/35047129

Structure of the bacteriophage φ29 DNA packaging motor. / Simpson, Alan A.; Tao, Yizhi; Leiman, Petr; Badasso, Mohammed O.; He, Yongning; Jardine, Paul J.; Olson, Norman H.; Morais, Marc; Grimes, Shelley; Anderson, Dwight L.; Baker, Timothy S.; Rossmann, Michael G.

In: Nature, Vol. 408, No. 6813, 07.12.2000, p. 745-750.

Research output: Contribution to journalArticle

Simpson, AA, Tao, Y, Leiman, P, Badasso, MO, He, Y, Jardine, PJ, Olson, NH, Morais, M, Grimes, S, Anderson, DL, Baker, TS & Rossmann, MG 2000, 'Structure of the bacteriophage φ29 DNA packaging motor', Nature, vol. 408, no. 6813, pp. 745-750. https://doi.org/10.1038/35047129
Simpson AA, Tao Y, Leiman P, Badasso MO, He Y, Jardine PJ et al. Structure of the bacteriophage φ29 DNA packaging motor. Nature. 2000 Dec 7;408(6813):745-750. https://doi.org/10.1038/35047129
Simpson, Alan A. ; Tao, Yizhi ; Leiman, Petr ; Badasso, Mohammed O. ; He, Yongning ; Jardine, Paul J. ; Olson, Norman H. ; Morais, Marc ; Grimes, Shelley ; Anderson, Dwight L. ; Baker, Timothy S. ; Rossmann, Michael G. / Structure of the bacteriophage φ29 DNA packaging motor. In: Nature. 2000 ; Vol. 408, No. 6813. pp. 745-750.
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