Structure of the cell-puncturing device of bacteriophage T4

Shuji Kanamaru, Petr Leiman, Victor A. Kostyuchenko, Paul R. Chipman, Vadim V. Mesyanzhinov, Fumio Arisaka, Michael G. Rossmann

Research output: Contribution to journalArticle

275 Citations (Scopus)

Abstract

Bacteriophage T4 has a very efficient mechanism for infecting cells. The key component of this process is the baseplate, located at the end of the phage tail, which regulates the interaction of the tail fibres and the DNA ejection machine. A complex of gene product (gp) 5 (63K) and gp27 (44K), the central part of the baseplate, is required to penetrate the outer cell membrane of Escherichia coli and to disrupt the intermembrane peptidoglycan layer, promoting subsequent entry of phage DNA into the host. We present here a crystal structure of the (gp5-gp27)3 321K complex, determined to 2.9 Å resolution and fitted into a cryo-electron microscopy map at 17 Å resolution of the baseplate-tail tube assembly. The carboxy-terminal domain of gp5 is a triple-stranded β-helix that forms an equilateral triangular prism, which acts as a membrane-puncturing needle. The middle lysozyme domain of gp5, situated on the periphery of the prism, serves to digest the peptidoglycan layer. The amino-terminal, antiparallel β-barrel domain of gp5 is inserted into a cylinder formed by three gp27 monomers, which may serve as a channel for DNA ejection.

Original languageEnglish (US)
Pages (from-to)553-557
Number of pages5
JournalNature
Volume415
Issue number6871
DOIs
StatePublished - Jan 31 2002
Externally publishedYes

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Bacteriophage T4
Peptidoglycan
Equipment and Supplies
Bacteriophages
DNA
Cryoelectron Microscopy
Muramidase
Needles
Cell Membrane
Escherichia coli
Membranes
Genes

ASJC Scopus subject areas

  • General

Cite this

Kanamaru, S., Leiman, P., Kostyuchenko, V. A., Chipman, P. R., Mesyanzhinov, V. V., Arisaka, F., & Rossmann, M. G. (2002). Structure of the cell-puncturing device of bacteriophage T4. Nature, 415(6871), 553-557. https://doi.org/10.1038/415553a

Structure of the cell-puncturing device of bacteriophage T4. / Kanamaru, Shuji; Leiman, Petr; Kostyuchenko, Victor A.; Chipman, Paul R.; Mesyanzhinov, Vadim V.; Arisaka, Fumio; Rossmann, Michael G.

In: Nature, Vol. 415, No. 6871, 31.01.2002, p. 553-557.

Research output: Contribution to journalArticle

Kanamaru, S, Leiman, P, Kostyuchenko, VA, Chipman, PR, Mesyanzhinov, VV, Arisaka, F & Rossmann, MG 2002, 'Structure of the cell-puncturing device of bacteriophage T4', Nature, vol. 415, no. 6871, pp. 553-557. https://doi.org/10.1038/415553a
Kanamaru S, Leiman P, Kostyuchenko VA, Chipman PR, Mesyanzhinov VV, Arisaka F et al. Structure of the cell-puncturing device of bacteriophage T4. Nature. 2002 Jan 31;415(6871):553-557. https://doi.org/10.1038/415553a
Kanamaru, Shuji ; Leiman, Petr ; Kostyuchenko, Victor A. ; Chipman, Paul R. ; Mesyanzhinov, Vadim V. ; Arisaka, Fumio ; Rossmann, Michael G. / Structure of the cell-puncturing device of bacteriophage T4. In: Nature. 2002 ; Vol. 415, No. 6871. pp. 553-557.
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