Abstract
Y-family DNA polymerases have proven to be remarkably diverse in their functions and in strategies for replicating through DNA lesions. The structure of yeast Rev1 ternary complex has revealed the most radical replication strategy, where the polymerase itself dictates the identity of the incoming nucleotide, as well as the identity of the templating base. We show here that many of the key elements of this highly unusual strategy are conserved between yeast and human Rev1, including the eviction of template G from the DNA helix and the pairing of incoming deoxycytidine 5'-triphosphate with a surrogate arginine residue. We also show that the catalytic core of human Rev1 is uniquely augmented by two large inserts, I1 and I2, wherein I1 extends > 20 Å away from the active site and may serve as a platform for protein-protein interactions specific for Rev1's role in translesion DNA synthesis in human cells, and I2 acts as a "flap" on the hydrophobic pocket accommodating template G. We suggest that these novel structural features are important for providing human Rev1 greater latitude in promoting efficient and error-free translesion DNA synthesis through the diverse array of bulky and potentially carcinogenic N2-deoxyguanosine DNA adducts in human cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 699-709 |
| Number of pages | 11 |
| Journal | Journal of Molecular Biology |
| Volume | 390 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jul 24 2009 |
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Keywords
- DNA polymerase
- DNA repair
- DNA replication
- N2-deoxyguanosine
- N2-dG
- Rev1
- Y-family polymerase
ASJC Scopus subject areas
- Molecular Biology
Cite this
Structure of the Human Rev1-DNA-dNTP Ternary Complex. / Swan, Michael K.; Johnson, Robert E.; Prakash, Louise; Prakash, Satya; Aggarwal, Aneel K.
In: Journal of Molecular Biology, Vol. 390, No. 4, 24.07.2009, p. 699-709.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Structure of the Human Rev1-DNA-dNTP Ternary Complex
AU - Swan, Michael K.
AU - Johnson, Robert E.
AU - Prakash, Louise
AU - Prakash, Satya
AU - Aggarwal, Aneel K.
PY - 2009/7/24
Y1 - 2009/7/24
N2 - Y-family DNA polymerases have proven to be remarkably diverse in their functions and in strategies for replicating through DNA lesions. The structure of yeast Rev1 ternary complex has revealed the most radical replication strategy, where the polymerase itself dictates the identity of the incoming nucleotide, as well as the identity of the templating base. We show here that many of the key elements of this highly unusual strategy are conserved between yeast and human Rev1, including the eviction of template G from the DNA helix and the pairing of incoming deoxycytidine 5'-triphosphate with a surrogate arginine residue. We also show that the catalytic core of human Rev1 is uniquely augmented by two large inserts, I1 and I2, wherein I1 extends > 20 Å away from the active site and may serve as a platform for protein-protein interactions specific for Rev1's role in translesion DNA synthesis in human cells, and I2 acts as a "flap" on the hydrophobic pocket accommodating template G. We suggest that these novel structural features are important for providing human Rev1 greater latitude in promoting efficient and error-free translesion DNA synthesis through the diverse array of bulky and potentially carcinogenic N2-deoxyguanosine DNA adducts in human cells.
AB - Y-family DNA polymerases have proven to be remarkably diverse in their functions and in strategies for replicating through DNA lesions. The structure of yeast Rev1 ternary complex has revealed the most radical replication strategy, where the polymerase itself dictates the identity of the incoming nucleotide, as well as the identity of the templating base. We show here that many of the key elements of this highly unusual strategy are conserved between yeast and human Rev1, including the eviction of template G from the DNA helix and the pairing of incoming deoxycytidine 5'-triphosphate with a surrogate arginine residue. We also show that the catalytic core of human Rev1 is uniquely augmented by two large inserts, I1 and I2, wherein I1 extends > 20 Å away from the active site and may serve as a platform for protein-protein interactions specific for Rev1's role in translesion DNA synthesis in human cells, and I2 acts as a "flap" on the hydrophobic pocket accommodating template G. We suggest that these novel structural features are important for providing human Rev1 greater latitude in promoting efficient and error-free translesion DNA synthesis through the diverse array of bulky and potentially carcinogenic N2-deoxyguanosine DNA adducts in human cells.
KW - DNA polymerase
KW - DNA repair
KW - DNA replication
KW - N2-deoxyguanosine
KW - N2-dG
KW - Rev1
KW - Y-family polymerase
UR - http://www.scopus.com/inward/record.url?scp=67649381629&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67649381629&partnerID=8YFLogxK
U2 - 10.1016/j.jmb.2009.05.026
DO - 10.1016/j.jmb.2009.05.026
M3 - Article
C2 - 19464298
AN - SCOPUS:67649381629
VL - 390
SP - 699
EP - 709
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 4
ER -