Structure of the intracellular defective viral RNAs of defective interfering particles of mouse hepatitis virus

Shinji Makino, N. Fujioka, K. Fujiwara

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The intracellular defective RNAs generated during high-multiplicity serial passages of mouse hepatitis virus JHM strain on DBT cells were examined. Seven novel species of single-stranded polyadenylic acid-containing defective RNAs were identified from passages 3 through 22. The largest of these RNAs, DIssA (molecular weight [mw], 5.2 x 106), is identical to the genomic RNA packaged in the defective interfering particles produced from these cells. Other RNA species, DIssB1 (mw, 1.9 x 106 to 1.6 x 106), DIssB2 (mw, 1.6 x 106), DIssC (mw, 2.8 x 106), DIssD (mw, 0.82 x 106), DIssE (mw, 0.78 x 106), and DIssF (mw, 1.3 x 106) were detected at different passage levels. RNase T1-resistant oligonucleotide fingerprinting demonstrated that all these RNAs were related and had multiple deletions of the genomic sequences. They contained different subsets of the genomic sequences from those of the standard intracellular mRNAs of nondefective mouse hepatitis virus JHM strain. Thus these novel intracellular viral RNAs were identified as defective interfering RNAs of mouse hepatitis virus JHM strain. The synthesis of six of the seven normal mRNA species specific to mouse hepatitis virus JHM strain was completely inhibited when cells were infected with viruses of late-passage levels. However, the synthesis of RNA7 and its product, viral nucleoprotein, was not significantly altered in late passages. The possible mechanism for the generation of defective interfering RNAs was discussed.

Original languageEnglish (US)
Pages (from-to)329-336
Number of pages8
JournalJournal of Virology
Volume54
Issue number2
StatePublished - 1985
Externally publishedYes

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Murine hepatitis virus
Defective Viruses
Viral RNA
RNA
Molecular Weight
molecular weight
genomics
Ribonuclease T1
Serial Passage
Messenger RNA
Poly A
Nucleoproteins
Sequence Deletion
nucleoproteins
synthesis
Oligonucleotides
cells
oligonucleotides

ASJC Scopus subject areas

  • Immunology

Cite this

Structure of the intracellular defective viral RNAs of defective interfering particles of mouse hepatitis virus. / Makino, Shinji; Fujioka, N.; Fujiwara, K.

In: Journal of Virology, Vol. 54, No. 2, 1985, p. 329-336.

Research output: Contribution to journalArticle

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