Studies in neuronal ceroid-lipofuscinosis

Heterogenous nature of neuronal autofluorescent lipopigments

H. S. Garg, Y. C. Awasthi, Satish Srivastava

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A method has been developed for the complete extraction and fractionation of the autofluorescent lipopigments present in the neuronal tissue of Batten-Spielmeyer-Vogt (BSV) patient and normal subject, respectively. The fluorescent lipopigments that were about three-fold higher in BSV patient's brain as compared with normal subjects were separated into at least 7 fluorometrically and chromatographically distinct fractions by thin layer chromatography (TLC). Although the fluorescent lipopigments of the brain tissue of BSV patient and normal subjects showed identical pattern of separation on TLC, the fluorescence spectra of the individual lipopigment fractions from BSV patient was different from those of the corresponding fractions from the normal subject. The ultraviolet spectral studies with individual fluorescent lipopigment fractions indicated the presence of highly unsaturated conjugated chromophore.

Original languageEnglish (US)
Pages (from-to)771-783
Number of pages13
JournalJournal of Neuroscience Research
Volume6
Issue number6
StatePublished - 1981

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Neuronal Ceroid-Lipofuscinoses
Thin Layer Chromatography
Brain
Fluorescence
lipopigments

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Studies in neuronal ceroid-lipofuscinosis : Heterogenous nature of neuronal autofluorescent lipopigments. / Garg, H. S.; Awasthi, Y. C.; Srivastava, Satish.

In: Journal of Neuroscience Research, Vol. 6, No. 6, 1981, p. 771-783.

Research output: Contribution to journalArticle

Garg, H. S. ; Awasthi, Y. C. ; Srivastava, Satish. / Studies in neuronal ceroid-lipofuscinosis : Heterogenous nature of neuronal autofluorescent lipopigments. In: Journal of Neuroscience Research. 1981 ; Vol. 6, No. 6. pp. 771-783.
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