Studies of cervical ripening in pregnant rats: Effects of various treatments

L. Shi, G. R. Saade, George Saade, R. E. Garfield

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The exact mechanisms that regulate cervical softening or ripening during pregnancy are not completely understood. The aim of this study was to estimate the effects of various agents on cervical softening during pregnancy in rats. Cervical resistance was examined after treatment with nitric oxide (no) donors and inhibitors and different hormonal agents. Cervical resistance was significantly reduced (P < 0.05) in rats treated with the NO donors: sodium nitroprusside, molsidomine and prostaglandin E2. However, treatments with the NO synthase (NOS) inhibitors N(ω)-nitro-L-arginine methyl ester (L- NAME) and L-N6-1-iminoethyl-lysine (L-NIL), or the prostaglandin synthesis inhibitor, indomethacin, significantly increased resistance (P < 0.05). The antiprogesterone, onapristone, reduced cervical resistance and its effects were only partially blocked by the progesterone agonist, promegestone. Relaxin reduced cervical resistance and NOS inhibitors partially blocked the effect of relaxin. These studies demonstrate that NO regulates cervical ripening. Relaxin also softens the cervix and may act by stimulating NO synthesis. Progesterone seems important in the control of cervical ripening, but its role appears complex. NO and prostaglandin pathways may independently control ripening by acting in parallel or synergistically.

Original languageEnglish
Pages (from-to)382-389
Number of pages8
JournalMolecular Human Reproduction
Volume6
Issue number4
StatePublished - Apr 2000

Fingerprint

Cervical Ripening
Relaxin
Nitric Oxide Synthase
Progesterone
Promegestone
Molsidomine
Prostaglandin Antagonists
Pregnancy
Nitric Oxide Donors
NG-Nitroarginine Methyl Ester
Nitroprusside
Dinoprostone
Cervix Uteri
Indomethacin
Prostaglandins

Keywords

  • Cervical ripening
  • Cervix
  • Nitric oxide
  • Pregnancy
  • Prostaglandins

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Genetics
  • Developmental Biology
  • Embryology
  • Cell Biology

Cite this

Studies of cervical ripening in pregnant rats : Effects of various treatments. / Shi, L.; Saade, G. R.; Saade, George; Garfield, R. E.

In: Molecular Human Reproduction, Vol. 6, No. 4, 04.2000, p. 382-389.

Research output: Contribution to journalArticle

Shi, L. ; Saade, G. R. ; Saade, George ; Garfield, R. E. / Studies of cervical ripening in pregnant rats : Effects of various treatments. In: Molecular Human Reproduction. 2000 ; Vol. 6, No. 4. pp. 382-389.
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