Studies of growth regulation in a neuroendocrine cell line

Courtney M. Townsend; Jin Ishizuka; James C. Thompson

doi:10.3109/02841869309083900

Studies of growth regulation in a neuroendocrine cell line

Courtney M. Townsend, Jin Ishizuka, James C. Thompson

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44 Scopus citations

Abstract

Studies of growth-regulation of neuroendocrine cells have been hampered by a lack of suitable in vitro models. We established and have maintained a functioning human pancreatic carcinoid cell (BON) line. BON cells synthesize and secrete several growth factors. Among those, we have found that serotonin stimulates growth of BON cells through specific receptors linked to cyclic AMP pathway. In this study, effects of other growth factors on growth and serotonin release, and the effects of serotonin and TGF-β1 on the polyamine biosynthetic pathway, were examined. TGF-β1 inhibited both growth and serotonin release in a dose-dependent fashion. Basic FGF stimulated growth, but failed to affect serotonin release. Other peptide growth factors had no effect on either growth or serotonin release. Serotonin stimulated ODC enzyme activity, but TGF-β1 failed to affect ODC enzyme activity. These findings suggest that growth of neuroendocrine cells can be delicately regulated by their own products in an autocrine fashion.

Original language	English (US)
Pages (from-to)	125-130
Number of pages	6
Journal	Acta Oncologica
Volume	32
Issue number	2
DOIs	https://doi.org/10.3109/02841869309083900
State	Published - 1993
Externally published	Yes

ASJC Scopus subject areas

Hematology
Oncology
Radiology Nuclear Medicine and imaging

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title = "Studies of growth regulation in a neuroendocrine cell line",

abstract = "Studies of growth-regulation of neuroendocrine cells have been hampered by a lack of suitable in vitro models. We established and have maintained a functioning human pancreatic carcinoid cell (BON) line. BON cells synthesize and secrete several growth factors. Among those, we have found that serotonin stimulates growth of BON cells through specific receptors linked to cyclic AMP pathway. In this study, effects of other growth factors on growth and serotonin release, and the effects of serotonin and TGF-β1 on the polyamine biosynthetic pathway, were examined. TGF-β1 inhibited both growth and serotonin release in a dose-dependent fashion. Basic FGF stimulated growth, but failed to affect serotonin release. Other peptide growth factors had no effect on either growth or serotonin release. Serotonin stimulated ODC enzyme activity, but TGF-β1 failed to affect ODC enzyme activity. These findings suggest that growth of neuroendocrine cells can be delicately regulated by their own products in an autocrine fashion.",

author = "Townsend, {Courtney M.} and Jin Ishizuka and Thompson, {James C.}",

note = "Funding Information: The authors would like to thank Ms. Jell Hsieh and Ms. Karen R. Cerdas for their technical assistance and Mr. Bob Todd for his help in the preparation of this manuscript. This work was supported by grants from the National Institutes of Health (5R37 DKI 5241, POI DK35608). American Cancer Society (PDT-220), Walls Medical Research Foundation and John-Sealy Endowment Fund.",

year = "1993",

doi = "10.3109/02841869309083900",

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AU - Ishizuka, Jin

AU - Thompson, James C.

N1 - Funding Information: The authors would like to thank Ms. Jell Hsieh and Ms. Karen R. Cerdas for their technical assistance and Mr. Bob Todd for his help in the preparation of this manuscript. This work was supported by grants from the National Institutes of Health (5R37 DKI 5241, POI DK35608). American Cancer Society (PDT-220), Walls Medical Research Foundation and John-Sealy Endowment Fund.

PY - 1993

Y1 - 1993

N2 - Studies of growth-regulation of neuroendocrine cells have been hampered by a lack of suitable in vitro models. We established and have maintained a functioning human pancreatic carcinoid cell (BON) line. BON cells synthesize and secrete several growth factors. Among those, we have found that serotonin stimulates growth of BON cells through specific receptors linked to cyclic AMP pathway. In this study, effects of other growth factors on growth and serotonin release, and the effects of serotonin and TGF-β1 on the polyamine biosynthetic pathway, were examined. TGF-β1 inhibited both growth and serotonin release in a dose-dependent fashion. Basic FGF stimulated growth, but failed to affect serotonin release. Other peptide growth factors had no effect on either growth or serotonin release. Serotonin stimulated ODC enzyme activity, but TGF-β1 failed to affect ODC enzyme activity. These findings suggest that growth of neuroendocrine cells can be delicately regulated by their own products in an autocrine fashion.

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Studies of growth regulation in a neuroendocrine cell line

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ASJC Scopus subject areas

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