Studies of growth regulation in a neuroendocrine cell line

Courtney M. Townsend; Jin Ishizuka; James C. Thompson

doi:10.3109/02841869309083900

Studies of growth regulation in a neuroendocrine cell line

Courtney M. Townsend, Jin Ishizuka, James C. Thompson

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Studies of growth-regulation of neuroendocrine cells have been hampered by a lack of suitable in vitro models. We established and have maintained a functioning human pancreatic carcinoid cell (BON) line. BON cells synthesize and secrete several growth factors. Among those, we have found that serotonin stimulates growth of BON cells through specific receptors linked to cyclic AMP pathway. In this study, effects of other growth factors on growth and serotonin release, and the effects of serotonin and TGF-β1 on the polyamine biosynthetic pathway, were examined. TGF-β1 inhibited both growth and serotonin release in a dose-dependent fashion. Basic FGF stimulated growth, but failed to affect serotonin release. Other peptide growth factors had no effect on either growth or serotonin release. Serotonin stimulated ODC enzyme activity, but TGF-β1 failed to affect ODC enzyme activity. These findings suggest that growth of neuroendocrine cells can be delicately regulated by their own products in an autocrine fashion.

Original language	English (US)
Pages (from-to)	125-130
Number of pages	6
Journal	Acta Oncologica
Volume	32
Issue number	2
DOIs	https://doi.org/10.3109/02841869309083900
State	Published - 1993
Externally published	Yes

ASJC Scopus subject areas

Hematology
Oncology
Radiology Nuclear Medicine and imaging

Access to Document

10.3109/02841869309083900

Cite this

@article{dee700e26ece4e0e86ecf54e23cfdf65,

title = "Studies of growth regulation in a neuroendocrine cell line",

abstract = "Studies of growth-regulation of neuroendocrine cells have been hampered by a lack of suitable in vitro models. We established and have maintained a functioning human pancreatic carcinoid cell (BON) line. BON cells synthesize and secrete several growth factors. Among those, we have found that serotonin stimulates growth of BON cells through specific receptors linked to cyclic AMP pathway. In this study, effects of other growth factors on growth and serotonin release, and the effects of serotonin and TGF-β1 on the polyamine biosynthetic pathway, were examined. TGF-β1 inhibited both growth and serotonin release in a dose-dependent fashion. Basic FGF stimulated growth, but failed to affect serotonin release. Other peptide growth factors had no effect on either growth or serotonin release. Serotonin stimulated ODC enzyme activity, but TGF-β1 failed to affect ODC enzyme activity. These findings suggest that growth of neuroendocrine cells can be delicately regulated by their own products in an autocrine fashion.",

author = "Townsend, {Courtney M.} and Jin Ishizuka and Thompson, {James C.}",

note = "Funding Information: The authors would like to thank Ms. Jell Hsieh and Ms. Karen R. Cerdas for their technical assistance and Mr. Bob Todd for his help in the preparation of this manuscript. This work was supported by grants from the National Institutes of Health (5R37 DKI 5241, POI DK35608). American Cancer Society (PDT-220), Walls Medical Research Foundation and John-Sealy Endowment Fund.",

year = "1993",

doi = "10.3109/02841869309083900",

language = "English (US)",

volume = "32",

pages = "125--130",

journal = "Acta Oncologica",

issn = "0284-186X",

publisher = "Medical Journals Sweden AB",

number = "2",

}

TY - JOUR

T1 - Studies of growth regulation in a neuroendocrine cell line

AU - Townsend, Courtney M.

AU - Ishizuka, Jin

AU - Thompson, James C.

N1 - Funding Information: The authors would like to thank Ms. Jell Hsieh and Ms. Karen R. Cerdas for their technical assistance and Mr. Bob Todd for his help in the preparation of this manuscript. This work was supported by grants from the National Institutes of Health (5R37 DKI 5241, POI DK35608). American Cancer Society (PDT-220), Walls Medical Research Foundation and John-Sealy Endowment Fund.

PY - 1993

Y1 - 1993

N2 - Studies of growth-regulation of neuroendocrine cells have been hampered by a lack of suitable in vitro models. We established and have maintained a functioning human pancreatic carcinoid cell (BON) line. BON cells synthesize and secrete several growth factors. Among those, we have found that serotonin stimulates growth of BON cells through specific receptors linked to cyclic AMP pathway. In this study, effects of other growth factors on growth and serotonin release, and the effects of serotonin and TGF-β1 on the polyamine biosynthetic pathway, were examined. TGF-β1 inhibited both growth and serotonin release in a dose-dependent fashion. Basic FGF stimulated growth, but failed to affect serotonin release. Other peptide growth factors had no effect on either growth or serotonin release. Serotonin stimulated ODC enzyme activity, but TGF-β1 failed to affect ODC enzyme activity. These findings suggest that growth of neuroendocrine cells can be delicately regulated by their own products in an autocrine fashion.

AB - Studies of growth-regulation of neuroendocrine cells have been hampered by a lack of suitable in vitro models. We established and have maintained a functioning human pancreatic carcinoid cell (BON) line. BON cells synthesize and secrete several growth factors. Among those, we have found that serotonin stimulates growth of BON cells through specific receptors linked to cyclic AMP pathway. In this study, effects of other growth factors on growth and serotonin release, and the effects of serotonin and TGF-β1 on the polyamine biosynthetic pathway, were examined. TGF-β1 inhibited both growth and serotonin release in a dose-dependent fashion. Basic FGF stimulated growth, but failed to affect serotonin release. Other peptide growth factors had no effect on either growth or serotonin release. Serotonin stimulated ODC enzyme activity, but TGF-β1 failed to affect ODC enzyme activity. These findings suggest that growth of neuroendocrine cells can be delicately regulated by their own products in an autocrine fashion.

UR - http://www.scopus.com/inward/record.url?scp=0027310390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027310390&partnerID=8YFLogxK

U2 - 10.3109/02841869309083900

DO - 10.3109/02841869309083900

M3 - Article

C2 - 8323753

AN - SCOPUS:0027310390

SN - 0284-186X

VL - 32

SP - 125

EP - 130

JO - Acta Oncologica

JF - Acta Oncologica

IS - 2

ER -

Studies of growth regulation in a neuroendocrine cell line

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this