Abstract
Studies of growth-regulation of neuroendocrine cells have been hampered by a lack of suitable in vitro models. We established and have maintained a functioning human pancreatic carcinoid cell (BON) line. BON cells synthesize and secrete several growth factors. Among those, we have found that serotonin stimulates growth of BON cells through specific receptors linked to cyclic AMP pathway. In this study, effects of other growth factors on growth and serotonin release, and the effects of serotonin and TGF-β1 on the polyamine biosynthetic pathway, were examined. TGF-β1 inhibited both growth and serotonin release in a dose-dependent fashion. Basic FGF stimulated growth, but failed to affect serotonin release. Other peptide growth factors had no effect on either growth or serotonin release. Serotonin stimulated ODC enzyme activity, but TGF-β1 failed to affect ODC enzyme activity. These findings suggest that growth of neuroendocrine cells can be delicately regulated by their own products in an autocrine fashion.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 125-130 |
| Number of pages | 6 |
| Journal | Acta Oncologica |
| Volume | 32 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1993 |
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ASJC Scopus subject areas
- Oncology
- Radiology Nuclear Medicine and imaging
- Hematology
Cite this
Studies of growth regulation in a neuroendocrine cell line. / Townsend, Courtney; Ishizuka, Jin; Thompson, James C.
In: Acta Oncologica, Vol. 32, No. 2, 1993, p. 125-130.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Studies of growth regulation in a neuroendocrine cell line
AU - Townsend, Courtney
AU - Ishizuka, Jin
AU - Thompson, James C.
PY - 1993
Y1 - 1993
N2 - Studies of growth-regulation of neuroendocrine cells have been hampered by a lack of suitable in vitro models. We established and have maintained a functioning human pancreatic carcinoid cell (BON) line. BON cells synthesize and secrete several growth factors. Among those, we have found that serotonin stimulates growth of BON cells through specific receptors linked to cyclic AMP pathway. In this study, effects of other growth factors on growth and serotonin release, and the effects of serotonin and TGF-β1 on the polyamine biosynthetic pathway, were examined. TGF-β1 inhibited both growth and serotonin release in a dose-dependent fashion. Basic FGF stimulated growth, but failed to affect serotonin release. Other peptide growth factors had no effect on either growth or serotonin release. Serotonin stimulated ODC enzyme activity, but TGF-β1 failed to affect ODC enzyme activity. These findings suggest that growth of neuroendocrine cells can be delicately regulated by their own products in an autocrine fashion.
AB - Studies of growth-regulation of neuroendocrine cells have been hampered by a lack of suitable in vitro models. We established and have maintained a functioning human pancreatic carcinoid cell (BON) line. BON cells synthesize and secrete several growth factors. Among those, we have found that serotonin stimulates growth of BON cells through specific receptors linked to cyclic AMP pathway. In this study, effects of other growth factors on growth and serotonin release, and the effects of serotonin and TGF-β1 on the polyamine biosynthetic pathway, were examined. TGF-β1 inhibited both growth and serotonin release in a dose-dependent fashion. Basic FGF stimulated growth, but failed to affect serotonin release. Other peptide growth factors had no effect on either growth or serotonin release. Serotonin stimulated ODC enzyme activity, but TGF-β1 failed to affect ODC enzyme activity. These findings suggest that growth of neuroendocrine cells can be delicately regulated by their own products in an autocrine fashion.
UR - http://www.scopus.com/inward/record.url?scp=0027310390&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027310390&partnerID=8YFLogxK
U2 - 10.3109/02841869309083900
DO - 10.3109/02841869309083900
M3 - Article
C2 - 8323753
AN - SCOPUS:0027310390
VL - 32
SP - 125
EP - 130
JO - Acta Oncologica
JF - Acta Oncologica
SN - 0284-186X
IS - 2
ER -