Studies of histidine as a suitable isoelectric buffer for tryptic digestion and isoelectric trapping fractionation followed by capillary electrophoresis-mass spectrometry for proteomic analysis

Stephanie M. Cologna, Brad J. Williams, William Russell, Pei Jing Pai, Gyula Vigh, David H. Russell

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The use of histidine as a protein digestion buffer followed by isoelectric trapping separations using "membrane separated wells for isoelectric focusing and trapping" (MSWIFT) and mass spectrometry (MS) analysis is described. Tryptic digestion of bovine serum albumin (BSA) performed in histidine buffered solutions yields similar amino acid sequence coverage values to those obtained using ammonium bicarbonate buffer. Time course studies suggest that histidine buffers provide faster migration of peptides from the loading compartment compared to digestions prepared in ammonium bicarbonate due to differences in conductivities of the two buffers. In addition, this sample preparation method and MSWIFT separations have been coupled with capillary electrophoresis (CE) and matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) as an alternative separation approach for proteomic studies. Tryptic peptides of ribosomal proteins in histidine are fractionated using MSWIFT followed by CE-MALDI-MS, which further illustrates the ability to couple fractions from a pI based separation device to CE-MS. Specifically, two-dimensional CE-MS plots provide a direct correlation between the numbers of basic residues within the peptide sequence displayed in charge-state trend lines. Combining MSWIFT and CE-MS provides added information regarding peptide sequence, specifically pI and in-solution charge state. Post-translational modifications can also be identified using this method.

Original languageEnglish (US)
Pages (from-to)8108-8114
Number of pages7
JournalAnalytical Chemistry
Volume83
Issue number21
DOIs
StatePublished - Nov 1 2011
Externally publishedYes

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Capillary electrophoresis
Fractionation
Histidine
Mass spectrometry
Buffers
Membranes
Peptides
Ionization
Desorption
Time and motion study
Lasers
Ribosomal Proteins
Bovine Serum Albumin
Proteomics
Amino Acids
Isoelectric Focusing
Proteins

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Studies of histidine as a suitable isoelectric buffer for tryptic digestion and isoelectric trapping fractionation followed by capillary electrophoresis-mass spectrometry for proteomic analysis. / Cologna, Stephanie M.; Williams, Brad J.; Russell, William; Pai, Pei Jing; Vigh, Gyula; Russell, David H.

In: Analytical Chemistry, Vol. 83, No. 21, 01.11.2011, p. 8108-8114.

Research output: Contribution to journalArticle

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