Studies on β-d-N-acethylhexosaminidase. Various isozymes in tissues of normal subjects and Sandhoff's disease patients

Satish Srivastava, John Wiktorowicz, Robert Klebe, Yogesh C. Awasthi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Hexosaminidase (EC 3.2.1.52) activity from human liver and kidney extract was completely precipitated by anti-hexosaminidase A antiserum and 80 to 90% by anti-hexosaminidase B antiserum. Immunologically distinct hexosaminidase "C" could not be detected in these tissues. The final fractions of hexosaminidase A eluted from DE-52 chromatography were resolved into several enzymatically active components by rechromatography. Compared to hexosaminidase A and B, these minor components are more anodal in polyacrylamide disc electrophoresis. The residual activity of hexosaminidase from liver and fibroblasts of patients with Sandhoff's disease has also been resolved into similar components. The enzyme activity of these more anodal hexosaminidase components was precipitated completely by anti-hexosaminidase A antiserum and partially by anti-hexosaminidase B antiserum. The minor, more anodal components probably represent hexosaminidase molecules having an altered ratio of subunits or the degradation products of hexosaminidase A.

Original languageEnglish (US)
Pages (from-to)428-436
Number of pages9
JournalBBA - Enzymology
Volume397
Issue number2
DOIs
StatePublished - Aug 26 1975

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Sandhoff Disease
Hexosaminidase A
Hexosaminidases
Hexosaminidase B
Isoenzymes
Immune Sera
Disc Electrophoresis
Liver Extracts
Human Activities
Chromatography
Fibroblasts
Kidney
Liver
Enzymes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Studies on β-d-N-acethylhexosaminidase. Various isozymes in tissues of normal subjects and Sandhoff's disease patients. / Srivastava, Satish; Wiktorowicz, John; Klebe, Robert; Awasthi, Yogesh C.

In: BBA - Enzymology, Vol. 397, No. 2, 26.08.1975, p. 428-436.

Research output: Contribution to journalArticle

Srivastava, Satish ; Wiktorowicz, John ; Klebe, Robert ; Awasthi, Yogesh C. / Studies on β-d-N-acethylhexosaminidase. Various isozymes in tissues of normal subjects and Sandhoff's disease patients. In: BBA - Enzymology. 1975 ; Vol. 397, No. 2. pp. 428-436.
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