Studies on γ glutamyl transpeptidase in human and rabbit erythrocytes

Satish Srivastava, Y. C. Awasthi, S. P. Miller, A. Yoshida, E. Beutler

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

γ Glutamyl transpeptidase transfers the γ glutamyl moiety of glutathione to a variety of acceptor amino acids. Through the operation of the γ glutamyl cyclotransferase cycle, this enzyme has been implicated in the transport of amino acids into cells, especially the cells of the proximal tubules of kidney. It has been reported to be present in rabbit erythrocytes. However, using white cell free preparations, the authors have not been able to demonstrate the presence of γ glutamyl transpeptidase in human or rabbit erythrocytes either by measuring the utilization of GSH or by following the formation of the product. 14C L methionine was used as acceptor amino acid, and the formation of γ glutamyl 14C L methionine was followed. Using similar conditions, the authors have been able to demonstrate the presence of γ glutamyl transpeptidase in human and rabbit leukocytes and in human kidney. In contrast to a previous report, the authors were unable to find the accumulation of 5 oxoproline, an intermediate of the γ glutamyl cyclotransferase pathway in human red cells incubated in Krebs Ringer solution. Immunologic studies demonstrated that human red cell membranes contained no protein antigenically similar to kidney γ glutamyl transpeptidase. Thus these studies indicated that in human and rabbit erythrocytes, the γ glutamyl transpeptidase cyclotransferase pathway was not operative.

Original languageEnglish (US)
Pages (from-to)645-650
Number of pages6
JournalBlood
Volume47
Issue number4
StatePublished - 1976

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gamma-Glutamyltransferase
Erythrocytes
Rabbits
Amino Acids
Methionine
Pyrrolidonecarboxylic Acid
Kidney
Cell membranes
Proximal Kidney Tubule
Glutathione
Cells
Leukocytes
Cell Membrane
Enzymes
Proteins

ASJC Scopus subject areas

  • Hematology

Cite this

Srivastava, S., Awasthi, Y. C., Miller, S. P., Yoshida, A., & Beutler, E. (1976). Studies on γ glutamyl transpeptidase in human and rabbit erythrocytes. Blood, 47(4), 645-650.

Studies on γ glutamyl transpeptidase in human and rabbit erythrocytes. / Srivastava, Satish; Awasthi, Y. C.; Miller, S. P.; Yoshida, A.; Beutler, E.

In: Blood, Vol. 47, No. 4, 1976, p. 645-650.

Research output: Contribution to journalArticle

Srivastava, S, Awasthi, YC, Miller, SP, Yoshida, A & Beutler, E 1976, 'Studies on γ glutamyl transpeptidase in human and rabbit erythrocytes', Blood, vol. 47, no. 4, pp. 645-650.
Srivastava S, Awasthi YC, Miller SP, Yoshida A, Beutler E. Studies on γ glutamyl transpeptidase in human and rabbit erythrocytes. Blood. 1976;47(4):645-650.
Srivastava, Satish ; Awasthi, Y. C. ; Miller, S. P. ; Yoshida, A. ; Beutler, E. / Studies on γ glutamyl transpeptidase in human and rabbit erythrocytes. In: Blood. 1976 ; Vol. 47, No. 4. pp. 645-650.
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