β (D) N Acetylhexosaminidase A and B were purified from human placenta. By extraction, ammonium sulfate precipitation, lyophilization, Sephadex G 200 filtration, and DEAE cellulose column chromatography, hexosaminidase A and hexosaminidase B were purified together. Hexosaminidase A was further purified by a second DEAE cellulose column chromatography followed by ECTEOLA cellulose column chromatography, Sephadex G 200 filtration, electrofocusing, and a third Sephadex G 200 filtration. Hexosaminidase B was further purified by CM cellulose column chromatography, electrofocusing, and Sephadex G 200 filtration. After the first Sephadex G 200 filtration hexosaminidase A and B were also purified by a second method. This method involved calcium phosphate gel, DEAE Sephadex, ECTEOLA cellulose, CM Sephadex and CM cellulose column chromatography, and preparative polyacrylamide disc electrophoresis. Purified hexosaminidase A and hexosaminidase B moved on polyacrylamide disc electrophoresis as single protein bands with virtually all of the enzyme activity. Hexosaminidase A and B in 10 mM phosphate buffer containing 0.1 M (NH4)2SO4 were stable at 4° for at least 4 mth. The isoelectric points of hexosaminidase A and hexosaminidase B were found to be pH 5.4 and 7.9, respectively. Placental hexosaminidases differed electrophoretically and antigenically from the liver and fibroblast enzymes. In contrast to the reports of others neuraminidase failed to convert heat labile hexosaminidase A to a heat stable form of enzyme resembling hexosaminidase B.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of Biological Chemistry|
|State||Published - 1974|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology