Studies on the attachment of Leishmania flagella to sand fly midgut epithelium.

A. Warburg, R. B. Tesh, D. McMahon-Pratt

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

An in vitro assay was developed to study the recognition mechanism for attachment of Leishmania flagella to sand fly midgut epithelium. Frozen sections of sand fly guts were incubated with flagella preparations, and probed with a flagella-specific monoclonal antibody. Tissue-specific adhesion of flagella to midgut epithelium was demonstrated by indirect immunofluorescence. None of the 13 sugars, screened to test for possible lectin-mediation, appeared to significantly inhibit the adhesion of flagella to gut sections. Similarly no inhibition was achieved by incubating flagella with pep 63 which inhibits the promastigote-macrophage recognition mechanism. Significant inhibition was attained by incubating flagella preparations with a monoclonal antibody which binds to a flagellar membrane-component. The possible relevance of the described mechanism for the biology of Leishmania in their sand fly hosts, is discussed.

Original languageEnglish (US)
Pages (from-to)613-617
Number of pages5
JournalJournal of Protozoology
Volume36
Issue number6
StatePublished - Nov 1989
Externally publishedYes

Fingerprint

Psychodidae
Flagella
Leishmania
Epithelium
Monoclonal Antibodies
Tissue Adhesions
Frozen Sections
Indirect Fluorescent Antibody Technique
Lectins
Macrophages
Membranes

ASJC Scopus subject areas

  • Parasitology

Cite this

Warburg, A., Tesh, R. B., & McMahon-Pratt, D. (1989). Studies on the attachment of Leishmania flagella to sand fly midgut epithelium. Journal of Protozoology, 36(6), 613-617.

Studies on the attachment of Leishmania flagella to sand fly midgut epithelium. / Warburg, A.; Tesh, R. B.; McMahon-Pratt, D.

In: Journal of Protozoology, Vol. 36, No. 6, 11.1989, p. 613-617.

Research output: Contribution to journalArticle

Warburg, A, Tesh, RB & McMahon-Pratt, D 1989, 'Studies on the attachment of Leishmania flagella to sand fly midgut epithelium.', Journal of Protozoology, vol. 36, no. 6, pp. 613-617.
Warburg, A. ; Tesh, R. B. ; McMahon-Pratt, D. / Studies on the attachment of Leishmania flagella to sand fly midgut epithelium. In: Journal of Protozoology. 1989 ; Vol. 36, No. 6. pp. 613-617.
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