Studies on the Attachment of Leishmania Flagella to Sand Fly Midgut Epithelium

ALON WARBURG, ROBERT B. TESH, DIANE McMAHON‐PRATT

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

An in vitro assay was developed to study the recognition mechanism for attachment of Leishmania flagella to sand fly midgut epithelium. Frozen sections of sand fly guts were incubated with Hagella preparations, and probed with a flagella‐specific monoclonal antibody. Tissue‐specific adhesion of flagella to midgut epithelium was demonstrated by indirect immunofluorescence. None of the 13 sugars, screened to test for possible lectin‐mediation, appeared to significantly inhibit the adhesion of flagella to gut sections. Similarly no inhibition was achieved by incubating flagella with pep 63 which inhibits the promastigote‐macrophage recognition mechanism. Significant inhibition was attained by incubating flagella preparations with a monoclonal antibody which binds to a flagellar membrane‐component. The possible relevance of the described mechanism for the biology of Leishmania in their sand fly hosts, is discussed.

Original languageEnglish (US)
Pages (from-to)613-617
Number of pages5
JournalThe Journal of Protozoology
Volume36
Issue number6
DOIs
StatePublished - Nov 1989

Keywords

  • Insect vector
  • lectin binding
  • monoclonal antibody

ASJC Scopus subject areas

  • Parasitology

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