Abstract
An in vitro assay was developed to study the recognition mechanism for attachment of Leishmania flagella to sand fly midgut epithelium. Frozen sections of sand fly guts were incubated with flagella preparations, and probed with a flagella-specific monoclonal antibody. Tissue-specific adhesion of flagella to midgut epithelium was demonstrated by indirect immunofluorescence. None of the 13 sugars, screened to test for possible lectin-mediation, appeared to significantly inhibit the adhesion of flagella to gut sections. Similarly no inhibition was achieved by incubating flagella with pep 63 which inhibits the promastigote-macrophage recognition mechanism. Significant inhibition was attained by incubating flagella preparations with a monoclonal antibody which binds to a flagellar membrane-component. The possible relevance of the described mechanism for the biology of Leishmania in their sand fly hosts, is discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 613-617 |
| Number of pages | 5 |
| Journal | Journal of Protozoology |
| Volume | 36 |
| Issue number | 6 |
| State | Published - Nov 1989 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Parasitology
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Studies on the attachment of Leishmania flagella to sand fly midgut epithelium. / Warburg, A.; Tesh, R. B.; McMahon-Pratt, D.
In: Journal of Protozoology, Vol. 36, No. 6, 11.1989, p. 613-617.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Studies on the attachment of Leishmania flagella to sand fly midgut epithelium.
AU - Warburg, A.
AU - Tesh, R. B.
AU - McMahon-Pratt, D.
PY - 1989/11
Y1 - 1989/11
N2 - An in vitro assay was developed to study the recognition mechanism for attachment of Leishmania flagella to sand fly midgut epithelium. Frozen sections of sand fly guts were incubated with flagella preparations, and probed with a flagella-specific monoclonal antibody. Tissue-specific adhesion of flagella to midgut epithelium was demonstrated by indirect immunofluorescence. None of the 13 sugars, screened to test for possible lectin-mediation, appeared to significantly inhibit the adhesion of flagella to gut sections. Similarly no inhibition was achieved by incubating flagella with pep 63 which inhibits the promastigote-macrophage recognition mechanism. Significant inhibition was attained by incubating flagella preparations with a monoclonal antibody which binds to a flagellar membrane-component. The possible relevance of the described mechanism for the biology of Leishmania in their sand fly hosts, is discussed.
AB - An in vitro assay was developed to study the recognition mechanism for attachment of Leishmania flagella to sand fly midgut epithelium. Frozen sections of sand fly guts were incubated with flagella preparations, and probed with a flagella-specific monoclonal antibody. Tissue-specific adhesion of flagella to midgut epithelium was demonstrated by indirect immunofluorescence. None of the 13 sugars, screened to test for possible lectin-mediation, appeared to significantly inhibit the adhesion of flagella to gut sections. Similarly no inhibition was achieved by incubating flagella with pep 63 which inhibits the promastigote-macrophage recognition mechanism. Significant inhibition was attained by incubating flagella preparations with a monoclonal antibody which binds to a flagellar membrane-component. The possible relevance of the described mechanism for the biology of Leishmania in their sand fly hosts, is discussed.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024757889&partnerID=8YFLogxK
M3 - Article
VL - 36
SP - 613
EP - 617
JO - Journal of Eukaryotic Microbiology
JF - Journal of Eukaryotic Microbiology
SN - 1066-5234
IS - 6
ER -