Studies on the cyclic AMP response to prostaglandin in human lymphocytes

James Goodwin, Shirley Bromberg, Ronald P. Messner

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

It is generally thought that cyclic AMP acts as the second messenger for prostaglandin E in human lymphocytes. We have recently found that the mitogen-induced proliferation of human lymphocytes is no longer inhibited by PGE2 if the lymphocytes are preincubated overnight prior to the addition of mitogens and PGE2. In this paper we report that lymphocytes also lose their cyclic AMP response to mitogens after preincubation. The loss of sensitivity to PGE with preincubation can be blocked by cyclohexamide (25 μg/ml). Indomethacin (1 μg/ml) partially blocked the loss of sensitivity, but removal of the glass-adherent cells did not. Since either manipulation effectively stops prostaglandin production in the preincubation cultures, it would appear that indomethacin prevented the loss of sensitivity to PGE2 by a mechanism other than inhibition of PG synthetase. The addition of phytohemagglutinin to the preincubation cultures also blocked the loss of sensitivity to PGE2.

Original languageEnglish (US)
Pages (from-to)298-307
Number of pages10
JournalCellular Immunology
Volume60
Issue number2
DOIs
StatePublished - May 15 1981
Externally publishedYes

Fingerprint

Dinoprostone
Cyclic AMP
Prostaglandins
Mitogens
Lymphocytes
Prostaglandins E
Indomethacin
Second Messenger Systems
Phytohemagglutinins
Ligases
Glass

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Studies on the cyclic AMP response to prostaglandin in human lymphocytes. / Goodwin, James; Bromberg, Shirley; Messner, Ronald P.

In: Cellular Immunology, Vol. 60, No. 2, 15.05.1981, p. 298-307.

Research output: Contribution to journalArticle

Goodwin, James ; Bromberg, Shirley ; Messner, Ronald P. / Studies on the cyclic AMP response to prostaglandin in human lymphocytes. In: Cellular Immunology. 1981 ; Vol. 60, No. 2. pp. 298-307.
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