Studies on the poxvirus cotia

J. J. Esposito, E. L. Palmer, E. C. Borden, A. K. Harrison, J. F. Obijeski, F. A. Murphy

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The poxvirus Cotia was studied by electron microscopy and by serological and biochemical analyses. Thin-sectioned preparations of infected Vero cells indicated that Cotia virus morphogenesis was similar to other mammalian poxviruses; unique filamentous structures and inclusion matrices were apparent in the cytoplasm. Complement fixation tests that included purified Cotia virions showed a reciprocal cross-reaction with rabbit myxoma virus and no cross-reaction with vaccinia virus. Serological results coupled with gradient polyacrylamide gel electropherograms of the structural proteins of purified Cotia, vaccinia, myxoma and fibroma viruses suggested that Cotia virus was similar to the latter two viruses. Agarose gel electropherograms of cleavage fragments of each of these virus DNAs digested with three separate restriction endonucleases showed that each of these viruses had a unique DNA gel profile.

Original languageEnglish (US)
Pages (from-to)37-46
Number of pages10
JournalJournal of General Virology
Volume47
Issue number1
StatePublished - 1980

Fingerprint

Poxviridae
Myxoma virus
Viruses
Cross Reactions
Gels
Vaccinia
Complement Fixation Tests
Vero Cells
DNA Viruses
Fibroma
Vaccinia virus
DNA Restriction Enzymes
Morphogenesis
Virion
Sepharose
Electron Microscopy
Cytoplasm
Rabbits
DNA
Proteins

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Esposito, J. J., Palmer, E. L., Borden, E. C., Harrison, A. K., Obijeski, J. F., & Murphy, F. A. (1980). Studies on the poxvirus cotia. Journal of General Virology, 47(1), 37-46.

Studies on the poxvirus cotia. / Esposito, J. J.; Palmer, E. L.; Borden, E. C.; Harrison, A. K.; Obijeski, J. F.; Murphy, F. A.

In: Journal of General Virology, Vol. 47, No. 1, 1980, p. 37-46.

Research output: Contribution to journalArticle

Esposito, JJ, Palmer, EL, Borden, EC, Harrison, AK, Obijeski, JF & Murphy, FA 1980, 'Studies on the poxvirus cotia', Journal of General Virology, vol. 47, no. 1, pp. 37-46.
Esposito JJ, Palmer EL, Borden EC, Harrison AK, Obijeski JF, Murphy FA. Studies on the poxvirus cotia. Journal of General Virology. 1980;47(1):37-46.
Esposito, J. J. ; Palmer, E. L. ; Borden, E. C. ; Harrison, A. K. ; Obijeski, J. F. ; Murphy, F. A. / Studies on the poxvirus cotia. In: Journal of General Virology. 1980 ; Vol. 47, No. 1. pp. 37-46.
@article{f73d30f2a5b74745a77b1408847ff2dd,
title = "Studies on the poxvirus cotia",
abstract = "The poxvirus Cotia was studied by electron microscopy and by serological and biochemical analyses. Thin-sectioned preparations of infected Vero cells indicated that Cotia virus morphogenesis was similar to other mammalian poxviruses; unique filamentous structures and inclusion matrices were apparent in the cytoplasm. Complement fixation tests that included purified Cotia virions showed a reciprocal cross-reaction with rabbit myxoma virus and no cross-reaction with vaccinia virus. Serological results coupled with gradient polyacrylamide gel electropherograms of the structural proteins of purified Cotia, vaccinia, myxoma and fibroma viruses suggested that Cotia virus was similar to the latter two viruses. Agarose gel electropherograms of cleavage fragments of each of these virus DNAs digested with three separate restriction endonucleases showed that each of these viruses had a unique DNA gel profile.",
author = "Esposito, {J. J.} and Palmer, {E. L.} and Borden, {E. C.} and Harrison, {A. K.} and Obijeski, {J. F.} and Murphy, {F. A.}",
year = "1980",
language = "English (US)",
volume = "47",
pages = "37--46",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "1",

}

TY - JOUR

T1 - Studies on the poxvirus cotia

AU - Esposito, J. J.

AU - Palmer, E. L.

AU - Borden, E. C.

AU - Harrison, A. K.

AU - Obijeski, J. F.

AU - Murphy, F. A.

PY - 1980

Y1 - 1980

N2 - The poxvirus Cotia was studied by electron microscopy and by serological and biochemical analyses. Thin-sectioned preparations of infected Vero cells indicated that Cotia virus morphogenesis was similar to other mammalian poxviruses; unique filamentous structures and inclusion matrices were apparent in the cytoplasm. Complement fixation tests that included purified Cotia virions showed a reciprocal cross-reaction with rabbit myxoma virus and no cross-reaction with vaccinia virus. Serological results coupled with gradient polyacrylamide gel electropherograms of the structural proteins of purified Cotia, vaccinia, myxoma and fibroma viruses suggested that Cotia virus was similar to the latter two viruses. Agarose gel electropherograms of cleavage fragments of each of these virus DNAs digested with three separate restriction endonucleases showed that each of these viruses had a unique DNA gel profile.

AB - The poxvirus Cotia was studied by electron microscopy and by serological and biochemical analyses. Thin-sectioned preparations of infected Vero cells indicated that Cotia virus morphogenesis was similar to other mammalian poxviruses; unique filamentous structures and inclusion matrices were apparent in the cytoplasm. Complement fixation tests that included purified Cotia virions showed a reciprocal cross-reaction with rabbit myxoma virus and no cross-reaction with vaccinia virus. Serological results coupled with gradient polyacrylamide gel electropherograms of the structural proteins of purified Cotia, vaccinia, myxoma and fibroma viruses suggested that Cotia virus was similar to the latter two viruses. Agarose gel electropherograms of cleavage fragments of each of these virus DNAs digested with three separate restriction endonucleases showed that each of these viruses had a unique DNA gel profile.

UR - http://www.scopus.com/inward/record.url?scp=0018863395&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018863395&partnerID=8YFLogxK

M3 - Article

VL - 47

SP - 37

EP - 46

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 1

ER -