Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies.

L. T. Figueiredo, R. M. Nogueira, S. M. Cavalcanti, H. Schatzmayr, A. T. da Rosa

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This paper presents the evaluation of an enzyme immunoassay in which Mayaro virus-infected cultured cells are used as antigen (EIA-ICC) and an IgM antibody capture ELISA (MAC-ELISA) for Mayaro serologic diagnosis using 114 human sera obtained during a Mayaro outbreak occurred in Bolivia, in 1987. Results were compared with those obtained by haemagglutination-inhibition test (HAI). MAC-ELISA was the most sensitive technique for anti-Mayaro IgM detection. MAC-ELISA was twice as sensitive as IgM EIA-ICC. The data shows that MAC-ELISA is a practical and valid technique for diagnosis of recent Mayaro infection. IgG EIA-ICC showed high sensitivity and high specificity compared to HAI. The combination of anti-Mayaro IgG and IgM EIA-ICC results presented the highest sensitivity of the study. Anti-Mayaro IgG and IgM simultaneous detection by EIA-ICC can be used for recent infection diagnosis (in spite of a less sensitive IgM detection than by MAC-ELISA), for surveillance and sero-epidemiologic studies, and for studies of IgG and IgM responses to Mayaro infection.

Original languageEnglish (US)
Pages (from-to)303-307
Number of pages5
JournalMemorias do Instituto Oswaldo Cruz
Volume84
Issue number3
StatePublished - Jul 1989
Externally publishedYes

Fingerprint

Immunoenzyme Techniques
Viruses
Immunoglobulin M
Enzyme-Linked Immunosorbent Assay
Antibodies
Enzymes
Hemagglutination Inhibition Tests
Immunoglobulin G
Infection
Bolivia
Cells
Antigens
Disease Outbreaks
Epidemiologic Studies
Cultured Cells
anti-IgM
Sensitivity and Specificity
Serum
anti-IgG

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Virology
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Figueiredo, L. T., Nogueira, R. M., Cavalcanti, S. M., Schatzmayr, H., & da Rosa, A. T. (1989). Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies. Memorias do Instituto Oswaldo Cruz, 84(3), 303-307.

Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies. / Figueiredo, L. T.; Nogueira, R. M.; Cavalcanti, S. M.; Schatzmayr, H.; da Rosa, A. T.

In: Memorias do Instituto Oswaldo Cruz, Vol. 84, No. 3, 07.1989, p. 303-307.

Research output: Contribution to journalArticle

Figueiredo, LT, Nogueira, RM, Cavalcanti, SM, Schatzmayr, H & da Rosa, AT 1989, 'Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies.', Memorias do Instituto Oswaldo Cruz, vol. 84, no. 3, pp. 303-307.
Figueiredo LT, Nogueira RM, Cavalcanti SM, Schatzmayr H, da Rosa AT. Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies. Memorias do Instituto Oswaldo Cruz. 1989 Jul;84(3):303-307.
Figueiredo, L. T. ; Nogueira, R. M. ; Cavalcanti, S. M. ; Schatzmayr, H. ; da Rosa, A. T. / Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies. In: Memorias do Instituto Oswaldo Cruz. 1989 ; Vol. 84, No. 3. pp. 303-307.
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