Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies.

L. T. Figueiredo, R. M. Nogueira, S. M. Cavalcanti, H. Schatzmayr, A. T. da Rosa

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Abstract

This paper presents the evaluation of an enzyme immunoassay in which Mayaro virus-infected cultured cells are used as antigen (EIA-ICC) and an IgM antibody capture ELISA (MAC-ELISA) for Mayaro serologic diagnosis using 114 human sera obtained during a Mayaro outbreak occurred in Bolivia, in 1987. Results were compared with those obtained by haemagglutination-inhibition test (HAI). MAC-ELISA was the most sensitive technique for anti-Mayaro IgM detection. MAC-ELISA was twice as sensitive as IgM EIA-ICC. The data shows that MAC-ELISA is a practical and valid technique for diagnosis of recent Mayaro infection. IgG EIA-ICC showed high sensitivity and high specificity compared to HAI. The combination of anti-Mayaro IgG and IgM EIA-ICC results presented the highest sensitivity of the study. Anti-Mayaro IgG and IgM simultaneous detection by EIA-ICC can be used for recent infection diagnosis (in spite of a less sensitive IgM detection than by MAC-ELISA), for surveillance and sero-epidemiologic studies, and for studies of IgG and IgM responses to Mayaro infection.

Original languageEnglish (US)
Pages (from-to)303-307
Number of pages5
JournalMemórias do Instituto Oswaldo Cruz
Volume84
Issue number3
StatePublished - Jan 1 1989

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ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Figueiredo, L. T., Nogueira, R. M., Cavalcanti, S. M., Schatzmayr, H., & da Rosa, A. T. (1989). Study of two different enzyme immunoassays for the detection of Mayaro virus antibodies. Memórias do Instituto Oswaldo Cruz, 84(3), 303-307.