Subpollen particles: Carriers of allergenic proteins and oxidases

Attila Bacsi, Barun Choudhury, Nilesh Dharajiya, Sanjiv Sur, Istvan Boldogh

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Background: Pollen is known to induce allergic asthma in atopic individuals, although only a few inhaled pollen grains penetrate into the lower respiratory tract. Objective: We sought to provide evidence that subpollen particles (SPPs) of respirable size, possessing both antigenic and redox properties, are released from weed pollen grains and to test their role in allergic airway inflammation. Methods: The release of SPPs was analyzed by means of microscopic imaging and flow cytometry. The redox properties of SPPs and the SPP-mediated oxidative effect on epithelial cells were determined by using redox-sensitive probes and specific inhibitors. Western blotting and amino acid sequence analysis were used to examine the protein components of the SPP. The allergenic properties of the SPP were determined in a murine model of experimental asthma. Results: Ragweed pollen grains released 0.5 to 4.5 μm of SPPs on hydration. These contained Amb a 1, along with other allergenic proteins of ragweed pollen, and possessed nicotinamide adenine dinucleotide (reduced) or nicotinamide adenine dinucleotide phosphate (reduced) [NAD(P)H] oxidase activity. The SPPs significantly increased the levels of reactive oxygen species (ROS) in cultured cells and induced allergic airway inflammation in the experimental animals. Pretreatment of the SPPs with NAD(P)H oxidase inhibitors attenuated their capacity to increase ROS levels in the airway epithelial cells and subsequent airway inflammation. Conclusions: The allergenic potency of SPPs released from ragweed pollen grains is mediated in tandem by ROS generated by intrinsic NAD(P)H oxidases and antigenic proteins. Clinical implications: Severe clinical symptoms associated with seasonal asthma might be explained by immune responses to inhaled SPPs carrying allergenic proteins and ROS-producing NAD(P)H oxidases.

Original languageEnglish (US)
Pages (from-to)844-850
Number of pages7
JournalJournal of Allergy and Clinical Immunology
Volume118
Issue number4
DOIs
StatePublished - Oct 2006

Fingerprint

Pollen
Carrier Proteins
Oxidoreductases
NADP
Reactive Oxygen Species
Oxidation-Reduction
Asthma
Inflammation
Proteins
Epithelial Cells
Protein Sequence Analysis
Particle Size
Respiratory System
NAD
Cultured Cells
Flow Cytometry
Theoretical Models
Western Blotting
ragweed pollen

Keywords

  • inflammation
  • oxidative stress
  • Subpollen particles

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Subpollen particles : Carriers of allergenic proteins and oxidases. / Bacsi, Attila; Choudhury, Barun; Dharajiya, Nilesh; Sur, Sanjiv; Boldogh, Istvan.

In: Journal of Allergy and Clinical Immunology, Vol. 118, No. 4, 10.2006, p. 844-850.

Research output: Contribution to journalArticle

Bacsi, Attila ; Choudhury, Barun ; Dharajiya, Nilesh ; Sur, Sanjiv ; Boldogh, Istvan. / Subpollen particles : Carriers of allergenic proteins and oxidases. In: Journal of Allergy and Clinical Immunology. 2006 ; Vol. 118, No. 4. pp. 844-850.
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