TY - JOUR
T1 - Substance P and related peptides in porcine cortex
T2 - Whole tissue and nuclear localization
AU - Nilsson, Carol L.
AU - Brodin, Ernst
AU - Ekman, Rolf
N1 - Funding Information:
This work was supported by grants from Stiftelsen Söderström-Königska Sjukhemmet, the Swedish Society for Medical Research and the Swedish Medical Research Council (07517 and 06836).
PY - 1998/3/20
Y1 - 1998/3/20
N2 - A large portion of the knowledge that has been gathered on the distribution of neuropeptides in neural tissues is based on findings obtained with immunocytochemistry and radioimmunoassay. However, these methods give limited structural information about the peptides being studied. Using porcine cortex as a model tissue, we combined immunoaffinity chromatography with reversed-phase high-performance liquid chromatography, radioimmunoassay, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF-MS). We determined the molecular nature of the peptides contributing to the substance P-like immunoreactivity measured in extracts of whole tissue and cell nuclei. In addition to substance P(1-11), other peptides were extracted using this protocol. The presence of SP(1-11) was confirmed through post-source decay analysis. These results illustrate the usefulness of MALDI-TOF-MS in the characterization of neuropeptides from biological tissues.
AB - A large portion of the knowledge that has been gathered on the distribution of neuropeptides in neural tissues is based on findings obtained with immunocytochemistry and radioimmunoassay. However, these methods give limited structural information about the peptides being studied. Using porcine cortex as a model tissue, we combined immunoaffinity chromatography with reversed-phase high-performance liquid chromatography, radioimmunoassay, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF-MS). We determined the molecular nature of the peptides contributing to the substance P-like immunoreactivity measured in extracts of whole tissue and cell nuclei. In addition to substance P(1-11), other peptides were extracted using this protocol. The presence of SP(1-11) was confirmed through post-source decay analysis. These results illustrate the usefulness of MALDI-TOF-MS in the characterization of neuropeptides from biological tissues.
KW - Immunoaffinity chromatography
KW - Peptides
KW - Substance P
UR - http://www.scopus.com/inward/record.url?scp=0032549803&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032549803&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(97)00930-8
DO - 10.1016/S0021-9673(97)00930-8
M3 - Article
C2 - 9561751
AN - SCOPUS:0032549803
VL - 800
SP - 21
EP - 27
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 1
ER -