Abstract
A large portion of the knowledge that has been gathered on the distribution of neuropeptides in neural tissues is based on findings obtained with immunocytochemistry and radioimmunoassay. However, these methods give limited structural information about the peptides being studied. Using porcine cortex as a model tissue, we combined immunoaffinity chromatography with reversed-phase high-performance liquid chromatography, radioimmunoassay, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF-MS). We determined the molecular nature of the peptides contributing to the substance P-like immunoreactivity measured in extracts of whole tissue and cell nuclei. In addition to substance P(1-11), other peptides were extracted using this protocol. The presence of SP(1-11) was confirmed through post-source decay analysis. These results illustrate the usefulness of MALDI-TOF-MS in the characterization of neuropeptides from biological tissues.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 21-27 |
| Number of pages | 7 |
| Journal | Journal of Chromatography A |
| Volume | 800 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 20 1998 |
| Externally published | Yes |
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Keywords
- Immunoaffinity chromatography
- Peptides
- Substance P
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Substance P and related peptides in porcine cortex : Whole tissue and nuclear localization. / Nilsson, Carol L.; Brodin, Ernst; Ekman, Rolf.
In: Journal of Chromatography A, Vol. 800, No. 1, 20.03.1998, p. 21-27.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Substance P and related peptides in porcine cortex
T2 - Whole tissue and nuclear localization
AU - Nilsson, Carol L.
AU - Brodin, Ernst
AU - Ekman, Rolf
PY - 1998/3/20
Y1 - 1998/3/20
N2 - A large portion of the knowledge that has been gathered on the distribution of neuropeptides in neural tissues is based on findings obtained with immunocytochemistry and radioimmunoassay. However, these methods give limited structural information about the peptides being studied. Using porcine cortex as a model tissue, we combined immunoaffinity chromatography with reversed-phase high-performance liquid chromatography, radioimmunoassay, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF-MS). We determined the molecular nature of the peptides contributing to the substance P-like immunoreactivity measured in extracts of whole tissue and cell nuclei. In addition to substance P(1-11), other peptides were extracted using this protocol. The presence of SP(1-11) was confirmed through post-source decay analysis. These results illustrate the usefulness of MALDI-TOF-MS in the characterization of neuropeptides from biological tissues.
AB - A large portion of the knowledge that has been gathered on the distribution of neuropeptides in neural tissues is based on findings obtained with immunocytochemistry and radioimmunoassay. However, these methods give limited structural information about the peptides being studied. Using porcine cortex as a model tissue, we combined immunoaffinity chromatography with reversed-phase high-performance liquid chromatography, radioimmunoassay, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF-MS). We determined the molecular nature of the peptides contributing to the substance P-like immunoreactivity measured in extracts of whole tissue and cell nuclei. In addition to substance P(1-11), other peptides were extracted using this protocol. The presence of SP(1-11) was confirmed through post-source decay analysis. These results illustrate the usefulness of MALDI-TOF-MS in the characterization of neuropeptides from biological tissues.
KW - Immunoaffinity chromatography
KW - Peptides
KW - Substance P
UR - http://www.scopus.com/inward/record.url?scp=0032549803&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032549803&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(97)00930-8
DO - 10.1016/S0021-9673(97)00930-8
M3 - Article
VL - 800
SP - 21
EP - 27
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - 1
ER -