Substance P and related peptides in porcine cortex

Whole tissue and nuclear localization

Carol L. Nilsson, Ernst Brodin, Rolf Ekman

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A large portion of the knowledge that has been gathered on the distribution of neuropeptides in neural tissues is based on findings obtained with immunocytochemistry and radioimmunoassay. However, these methods give limited structural information about the peptides being studied. Using porcine cortex as a model tissue, we combined immunoaffinity chromatography with reversed-phase high-performance liquid chromatography, radioimmunoassay, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF-MS). We determined the molecular nature of the peptides contributing to the substance P-like immunoreactivity measured in extracts of whole tissue and cell nuclei. In addition to substance P(1-11), other peptides were extracted using this protocol. The presence of SP(1-11) was confirmed through post-source decay analysis. These results illustrate the usefulness of MALDI-TOF-MS in the characterization of neuropeptides from biological tissues.

Original languageEnglish (US)
Pages (from-to)21-27
Number of pages7
JournalJournal of Chromatography A
Volume800
Issue number1
DOIs
StatePublished - Mar 20 1998
Externally publishedYes

Fingerprint

Substance P
Swine
Tissue
Neuropeptides
Peptides
Radioimmunoassay
Mass Spectrometry
Lasers
Ionization
Mass spectrometry
Desorption
Tissue Extracts
Reverse-Phase Chromatography
Cell Nucleus
Chromatography
High performance liquid chromatography
Immunohistochemistry
High Pressure Liquid Chromatography
Cells

Keywords

  • Immunoaffinity chromatography
  • Peptides
  • Substance P

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Substance P and related peptides in porcine cortex : Whole tissue and nuclear localization. / Nilsson, Carol L.; Brodin, Ernst; Ekman, Rolf.

In: Journal of Chromatography A, Vol. 800, No. 1, 20.03.1998, p. 21-27.

Research output: Contribution to journalArticle

Nilsson, Carol L. ; Brodin, Ernst ; Ekman, Rolf. / Substance P and related peptides in porcine cortex : Whole tissue and nuclear localization. In: Journal of Chromatography A. 1998 ; Vol. 800, No. 1. pp. 21-27.
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