Substance P expression correlates with severity of diarrhea in cryptosporidiosis

Prema Robinson, Pablo C. Okhuysen, Cynthia L. Chappell, Joel V. Weinstock, Dorothy E. Lewis, Jeffrey K. Actor, A. Clinton White

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Cryptosporidiosis, caused by Cryptosporidium parvum, is self-limited in immunocompetent hosts but may cause chronic diarrhea in patients with acquired immunodeficiency syndrome (AIDS). Substance P (SP), a neuropeptide belonging to the tachykinin family, is expressed in gastrointestinal tract and can cause electrogenic chloride anion secretion. Therefore, we studied SP mRNA and protein expression in jejunal tissue samples of patients with AIDS with naturally occurring chronic cryptosporidiosis and healthy volunteers with mild cryptosporidiosis or asymptomatic infection after experimental C. parvum challenge. SP mRNA was associated with symptoms in cryptosporidiosis. SP protein levels were greater in symptomatic than asymptomatic volunteers. Similarly, greater expression of SP mRNA and protein were noted in patients with AIDS with chronic cryptosporidiosis versus immunocompetent volunteers with self-limited infection. This study demonstrates a direct correlation between SP levels and disease severity and may imply that SP plays a role in diarrhea mediation.

Original languageEnglish (US)
Pages (from-to)290-296
Number of pages7
JournalJournal of Infectious Diseases
Volume188
Issue number2
DOIs
StatePublished - Jul 15 2003
Externally publishedYes

Fingerprint

Cryptosporidiosis
Substance P
Diarrhea
Cryptosporidium parvum
Acquired Immunodeficiency Syndrome
Messenger RNA
Volunteers
Tachykinins
Asymptomatic Infections
Proteins
Neuropeptides
Anions
Gastrointestinal Tract
Chlorides
Healthy Volunteers
Infection

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Robinson, P., Okhuysen, P. C., Chappell, C. L., Weinstock, J. V., Lewis, D. E., Actor, J. K., & White, A. C. (2003). Substance P expression correlates with severity of diarrhea in cryptosporidiosis. Journal of Infectious Diseases, 188(2), 290-296. https://doi.org/10.1086/376836

Substance P expression correlates with severity of diarrhea in cryptosporidiosis. / Robinson, Prema; Okhuysen, Pablo C.; Chappell, Cynthia L.; Weinstock, Joel V.; Lewis, Dorothy E.; Actor, Jeffrey K.; White, A. Clinton.

In: Journal of Infectious Diseases, Vol. 188, No. 2, 15.07.2003, p. 290-296.

Research output: Contribution to journalArticle

Robinson, P, Okhuysen, PC, Chappell, CL, Weinstock, JV, Lewis, DE, Actor, JK & White, AC 2003, 'Substance P expression correlates with severity of diarrhea in cryptosporidiosis', Journal of Infectious Diseases, vol. 188, no. 2, pp. 290-296. https://doi.org/10.1086/376836
Robinson P, Okhuysen PC, Chappell CL, Weinstock JV, Lewis DE, Actor JK et al. Substance P expression correlates with severity of diarrhea in cryptosporidiosis. Journal of Infectious Diseases. 2003 Jul 15;188(2):290-296. https://doi.org/10.1086/376836
Robinson, Prema ; Okhuysen, Pablo C. ; Chappell, Cynthia L. ; Weinstock, Joel V. ; Lewis, Dorothy E. ; Actor, Jeffrey K. ; White, A. Clinton. / Substance P expression correlates with severity of diarrhea in cryptosporidiosis. In: Journal of Infectious Diseases. 2003 ; Vol. 188, No. 2. pp. 290-296.
@article{ea6dcbc9981340e5b99d9e1bb6d10a1e,
title = "Substance P expression correlates with severity of diarrhea in cryptosporidiosis",
abstract = "Cryptosporidiosis, caused by Cryptosporidium parvum, is self-limited in immunocompetent hosts but may cause chronic diarrhea in patients with acquired immunodeficiency syndrome (AIDS). Substance P (SP), a neuropeptide belonging to the tachykinin family, is expressed in gastrointestinal tract and can cause electrogenic chloride anion secretion. Therefore, we studied SP mRNA and protein expression in jejunal tissue samples of patients with AIDS with naturally occurring chronic cryptosporidiosis and healthy volunteers with mild cryptosporidiosis or asymptomatic infection after experimental C. parvum challenge. SP mRNA was associated with symptoms in cryptosporidiosis. SP protein levels were greater in symptomatic than asymptomatic volunteers. Similarly, greater expression of SP mRNA and protein were noted in patients with AIDS with chronic cryptosporidiosis versus immunocompetent volunteers with self-limited infection. This study demonstrates a direct correlation between SP levels and disease severity and may imply that SP plays a role in diarrhea mediation.",
author = "Prema Robinson and Okhuysen, {Pablo C.} and Chappell, {Cynthia L.} and Weinstock, {Joel V.} and Lewis, {Dorothy E.} and Actor, {Jeffrey K.} and White, {A. Clinton}",
year = "2003",
month = "7",
day = "15",
doi = "10.1086/376836",
language = "English (US)",
volume = "188",
pages = "290--296",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - Substance P expression correlates with severity of diarrhea in cryptosporidiosis

AU - Robinson, Prema

AU - Okhuysen, Pablo C.

AU - Chappell, Cynthia L.

AU - Weinstock, Joel V.

AU - Lewis, Dorothy E.

AU - Actor, Jeffrey K.

AU - White, A. Clinton

PY - 2003/7/15

Y1 - 2003/7/15

N2 - Cryptosporidiosis, caused by Cryptosporidium parvum, is self-limited in immunocompetent hosts but may cause chronic diarrhea in patients with acquired immunodeficiency syndrome (AIDS). Substance P (SP), a neuropeptide belonging to the tachykinin family, is expressed in gastrointestinal tract and can cause electrogenic chloride anion secretion. Therefore, we studied SP mRNA and protein expression in jejunal tissue samples of patients with AIDS with naturally occurring chronic cryptosporidiosis and healthy volunteers with mild cryptosporidiosis or asymptomatic infection after experimental C. parvum challenge. SP mRNA was associated with symptoms in cryptosporidiosis. SP protein levels were greater in symptomatic than asymptomatic volunteers. Similarly, greater expression of SP mRNA and protein were noted in patients with AIDS with chronic cryptosporidiosis versus immunocompetent volunteers with self-limited infection. This study demonstrates a direct correlation between SP levels and disease severity and may imply that SP plays a role in diarrhea mediation.

AB - Cryptosporidiosis, caused by Cryptosporidium parvum, is self-limited in immunocompetent hosts but may cause chronic diarrhea in patients with acquired immunodeficiency syndrome (AIDS). Substance P (SP), a neuropeptide belonging to the tachykinin family, is expressed in gastrointestinal tract and can cause electrogenic chloride anion secretion. Therefore, we studied SP mRNA and protein expression in jejunal tissue samples of patients with AIDS with naturally occurring chronic cryptosporidiosis and healthy volunteers with mild cryptosporidiosis or asymptomatic infection after experimental C. parvum challenge. SP mRNA was associated with symptoms in cryptosporidiosis. SP protein levels were greater in symptomatic than asymptomatic volunteers. Similarly, greater expression of SP mRNA and protein were noted in patients with AIDS with chronic cryptosporidiosis versus immunocompetent volunteers with self-limited infection. This study demonstrates a direct correlation between SP levels and disease severity and may imply that SP plays a role in diarrhea mediation.

UR - http://www.scopus.com/inward/record.url?scp=0041814638&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0041814638&partnerID=8YFLogxK

U2 - 10.1086/376836

DO - 10.1086/376836

M3 - Article

VL - 188

SP - 290

EP - 296

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 2

ER -