Substitution of NS5 N-terminal domain of dengue virus type 2 RNA with type 4 domain caused impaired replication and emergence of adaptive mutants with enhanced fitness

Tadahisa Teramoto; Siwaporn Boonyasuppayakorn; Misty Handley; Kyung H. Choi; Radhakrishnan Padmanabhan

doi:10.1074/jbc.M114.584466

Substitution of NS5 N-terminal domain of dengue virus type 2 RNA with type 4 domain caused impaired replication and emergence of adaptive mutants with enhanced fitness

Tadahisa Teramoto
, Siwaporn Boonyasuppayakorn
, Misty Handley
, Kyung H. Choi
, Radhakrishnan Padmanabhan

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Background: The four dengue virus serotypes, evolved from a common ancestor, are global human pathogens. Results: Interserotypic substitution of the functional domain essential for 5′-capping was detrimental to RNA replication. Conclusion: The chimeric RNA gradually evolves replication fitness through adaptive mutations in genes encoding two replication proteins. Significance: This study provides a possible pathway for generating attenuated dengue virus vaccine.

Original language	English (US)
Pages (from-to)	22385-22400
Number of pages	16
Journal	Journal of Biological Chemistry
Volume	289
Issue number	32
DOIs	https://doi.org/10.1074/jbc.M114.584466
State	Published - Aug 8 2014
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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10.1074/jbc.M114.584466

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Substitution of NS5 N-terminal domain of dengue virus type 2 RNA with type 4 domain caused impaired replication and emergence of adaptive mutants with enhanced fitness. / Teramoto, Tadahisa; Boonyasuppayakorn, Siwaporn; Handley, Misty et al.
In: Journal of Biological Chemistry, Vol. 289, No. 32, 08.08.2014, p. 22385-22400.

Research output: Contribution to journal › Article › peer-review

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AU - Choi, Kyung H.

AU - Padmanabhan, Radhakrishnan

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Substitution of NS5 N-terminal domain of dengue virus type 2 RNA with type 4 domain caused impaired replication and emergence of adaptive mutants with enhanced fitness

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