Substrate specificity and screening of the integral membrane protease Pla

Anton Agarkov; Sadhana Chauhan; Pedro J. Lory; Scott R. Gilbertson; Vladimir L. Motin

doi:10.1016/j.bmcl.2007.09.104

Substrate specificity and screening of the integral membrane protease Pla

Anton Agarkov, Sadhana Chauhan, Pedro J. Lory, Scott R. Gilbertson, Vladimir L. Motin

Pathology

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

This paper reports a study to find small peptide substrates for the important virulence factor of Yersinia pestis, plasminogen activator, Pla. The method used to find small substrates for this protease is reported along with studies examining the ability of these peptides to inhibit activity of the enzyme. Through the use of parallel synthesis and positional scanning, small tripeptides were identified that are viable substrates for the protease.

Original language	English (US)
Pages (from-to)	427-431
Number of pages	5
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	18
Issue number	1
DOIs	https://doi.org/10.1016/j.bmcl.2007.09.104
State	Published - Jan 1 2008

Keywords

Parallel synthesis
Peptide inhibitor
Plasminogen activator
Positional scan
Protease substrate
Yersinia pestis

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmcl.2007.09.104

Cite this

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title = "Substrate specificity and screening of the integral membrane protease Pla",

abstract = "This paper reports a study to find small peptide substrates for the important virulence factor of Yersinia pestis, plasminogen activator, Pla. The method used to find small substrates for this protease is reported along with studies examining the ability of these peptides to inhibit activity of the enzyme. Through the use of parallel synthesis and positional scanning, small tripeptides were identified that are viable substrates for the protease.",

keywords = "Parallel synthesis, Peptide inhibitor, Plasminogen activator, Positional scan, Protease substrate, Yersinia pestis",

author = "Anton Agarkov and Sadhana Chauhan and Lory, {Pedro J.} and Gilbertson, {Scott R.} and Motin, {Vladimir L.}",

note = "Funding Information: This work was supported by the Region VI {\textquoteleft}Western{\textquoteright} RCE (NIH award 1-U54-AI-057156) grant, an award from the Sealy Center for Vaccine Development of the University of Texas Medical Branch, and the Robert A. Welch Foundation. We thank Dr. Robert R. Brubaker for providing the strains of Y. pestis and helpful discussions. ",

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AU - Lory, Pedro J.

AU - Gilbertson, Scott R.

AU - Motin, Vladimir L.

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AB - This paper reports a study to find small peptide substrates for the important virulence factor of Yersinia pestis, plasminogen activator, Pla. The method used to find small substrates for this protease is reported along with studies examining the ability of these peptides to inhibit activity of the enzyme. Through the use of parallel synthesis and positional scanning, small tripeptides were identified that are viable substrates for the protease.

KW - Parallel synthesis

KW - Peptide inhibitor

KW - Plasminogen activator

KW - Positional scan

KW - Protease substrate

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Substrate specificity and screening of the integral membrane protease Pla

Abstract

Keywords

ASJC Scopus subject areas

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