Substrate topography and size determine the fate of human embryonic stem cells to neuronal or glial lineage

Soneela Ankam, Mona Suryana, Lesley Y. Chan, Aung Aung Kywe Moe, Benjamin K.K. Teo, Jaslyn B.K. Law, Michael Sheetz, Hong Yee Low, Evelyn K.F. Yim

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Efficient derivation of neural cells from human embryonic stem cells (hESCs) remains an unmet need for the treatment of neurological disorders. The limiting factors for current methods include being labor-intensive, time-consuming and expensive. In this study, we hypothesize that the substrate topography, with optimal geometry and dimension, can modulate the neural fate of hESCs and enhance the efficiency of differentiation. A multi-architectural chip (MARC) containing fields of topographies varying in geometry and dimension was developed to facilitate high-throughput analysis of topography-induced neural differentiation in vitro. The hESCs were subjected to "direct differentiation", in which small clumps of undifferentiated hESCs were cultured directly without going through the stage of embryoid body formation, on the MARC with N2 and B27 supplements for 7 days. The gene and protein expression analysis indicated that the anisotropic patterns like gratings promoted neuronal differentiation of hESCs while the isotropic patterns like pillars and wells promoted the glial differentiation of hESCs. This study showed that optimal combination of topography and biochemical cues could shorten the differentiation period and allowed derivation of neurons bearing longer neurites that were aligned along the grating axis. The MARC platform would enable high-throughput screening of topographical substrates that could maximize the efficiency of neuronal differentiation from pluripotent stem cells.

Original languageEnglish (US)
Pages (from-to)4535-4545
Number of pages11
JournalActa Biomaterialia
Volume9
Issue number1
DOIs
StatePublished - Jan 1 2013
Externally publishedYes

Fingerprint

Stem cells
Neuroglia
Topography
Substrates
Bearings (structural)
Embryoid Bodies
Efficiency
Throughput
Pluripotent Stem Cells
Neurites
Nervous System Diseases
Geometry
Cues
Human Embryonic Stem Cells
Neurons
Screening
Genes
Gene Expression
Personnel
Proteins

Keywords

  • High-throughput screening
  • Multiarchitectural array chip
  • Nanoimprinting
  • Neuronal differentiation
  • Pluripotent stem cells

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Ankam, S., Suryana, M., Chan, L. Y., Moe, A. A. K., Teo, B. K. K., Law, J. B. K., ... Yim, E. K. F. (2013). Substrate topography and size determine the fate of human embryonic stem cells to neuronal or glial lineage. Acta Biomaterialia, 9(1), 4535-4545. https://doi.org/10.1016/j.actbio.2012.08.018

Substrate topography and size determine the fate of human embryonic stem cells to neuronal or glial lineage. / Ankam, Soneela; Suryana, Mona; Chan, Lesley Y.; Moe, Aung Aung Kywe; Teo, Benjamin K.K.; Law, Jaslyn B.K.; Sheetz, Michael; Low, Hong Yee; Yim, Evelyn K.F.

In: Acta Biomaterialia, Vol. 9, No. 1, 01.01.2013, p. 4535-4545.

Research output: Contribution to journalArticle

Ankam, S, Suryana, M, Chan, LY, Moe, AAK, Teo, BKK, Law, JBK, Sheetz, M, Low, HY & Yim, EKF 2013, 'Substrate topography and size determine the fate of human embryonic stem cells to neuronal or glial lineage', Acta Biomaterialia, vol. 9, no. 1, pp. 4535-4545. https://doi.org/10.1016/j.actbio.2012.08.018
Ankam, Soneela ; Suryana, Mona ; Chan, Lesley Y. ; Moe, Aung Aung Kywe ; Teo, Benjamin K.K. ; Law, Jaslyn B.K. ; Sheetz, Michael ; Low, Hong Yee ; Yim, Evelyn K.F. / Substrate topography and size determine the fate of human embryonic stem cells to neuronal or glial lineage. In: Acta Biomaterialia. 2013 ; Vol. 9, No. 1. pp. 4535-4545.
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