Subunit composition of strychnine-sensitive glycine receptors expressed by adult rat basolateral amygdala neurons

Brian A. McCool, Jeffrey Farroni

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

In neonatal rats, strychnine-sensitive glycine receptors are widely expressed in the spinal cord, brainstem and forebrain. During development, these 'neonatal' receptors are replaced by an adult isoform, the expression of which becomes restricted primarily to brain stem and spinal cord. Unlike most forebrain regions, functional strychnine-sensitive glycine receptors appear to persist within adult rat amygdala. However, the subunit composition of glycine receptors expressed by amygdala neurons and its relationship to the adult isoform in brain stem/spinal cord has not been defined precisely. In this report, we have utilized RT-PCR and single-cell RT-PCR to demonstrate that the 'neonatal' α2-subunit mRNA persists in adult rat amygdala neurons and is the predominant α-subunit. We further demonstrate that native amygdala glycine receptors are relatively insensitive to the receptor antagonist picrotoxin, suggesting that α2- and β-subunits may be present together in the same multisubunit complex. We further demonstrate that α2- and β-subunits cloned from adult rat amygdala can form functional channels when expressed in a heterologous system. Together, these studies highlight both the unique characteristics of strychnine-sensitive glycine receptors in the adult rat amygdala as well as the possibility that α2/β channels may represent the adult forebrain isoform of the strychnine-sensitive glycine receptor.

Original languageEnglish (US)
Pages (from-to)1082-1090
Number of pages9
JournalEuropean Journal of Neuroscience
Volume14
Issue number7
DOIs
StatePublished - 2001
Externally publishedYes

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Glycine Receptors
Strychnine
Amygdala
Neurons
Prosencephalon
Brain Stem
Spinal Cord
Protein Isoforms
Polymerase Chain Reaction
Basolateral Nuclear Complex
Messenger RNA

Keywords

  • Heterologous expression
  • Limbic forebrain
  • Picrotoxin
  • Single-cell RT-PCR

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Subunit composition of strychnine-sensitive glycine receptors expressed by adult rat basolateral amygdala neurons. / McCool, Brian A.; Farroni, Jeffrey.

In: European Journal of Neuroscience, Vol. 14, No. 7, 2001, p. 1082-1090.

Research output: Contribution to journalArticle

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