Subversion of pulmonary dendritic cell function by paramyxovirus infections

Antonieta Guerrero-Plata, Deepthi Kolli, Chao Hong, Antonella Casola, Roberto Garofalo

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Lower respiratory tract infections caused by the paramyxoviruses human metapneumovirus (hMPV) and respiratory syncytial virus (RSV) are characterized by short-lasting virus-specific immunity and often long-term airway morbidity, both of which may be the result of alterations in the Ag-presenting function of the lung which follow these infections. In this study, we investigated whether hMPV and RSV experimental infections alter the phenotype and function of dendritic cell (DC) subsets that are recruited to the lung. Characterization of lung DC trafficking demonstrated a differential recruitment of plasmacytoid DC (pDC), conventional DC (cDC), and IFN-producing killer DC to the lung and draining lymph nodes after hMPV and RSV infection. In vitro infection of lung DC indicated that in pDC, production of IFN-α, TNF-α, and CCL5 was induced only by hMPV, whereas CCL3 and CCL4 were induced by both viruses. In cDC, a similar repertoire of cytokines was induced by hMPV and RSV, except for IFN-β, which was not induced by RSV. The function of lung pDC was altered following hMPV or RSV infection in vivo, as we demonstrated a reduced capacity of lung pDC to produce IFN-α as well as other cytokines including IL-6, TNF-α, CCL2, CCL3, and CCL4 in response to TLR9 stimulation. Moreover, we observed an impaired capacity of cDC from infected mice to present Ag to CD4+ T cells, an effect that lasted beyond the acute phase of infection. Our findings suggest that acute paramyxovirus infections can alter the long-term immune function of pulmonary DC.

Original languageEnglish (US)
Pages (from-to)3072-3083
Number of pages12
JournalJournal of Immunology
Volume182
Issue number5
DOIs
StatePublished - Mar 1 2009

Fingerprint

Metapneumovirus
Dendritic Cells
Human respiratory syncytial virus
Lung
Respiratory Syncytial Virus Infections
Infection
Cytokines
Viruses
Lung Volume Measurements
Respiratory Syncytial Viruses
Respiratory Tract Infections
Immunity
Interleukin-6
Lymph Nodes
Morbidity
T-Lymphocytes
Phenotype

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Subversion of pulmonary dendritic cell function by paramyxovirus infections. / Guerrero-Plata, Antonieta; Kolli, Deepthi; Hong, Chao; Casola, Antonella; Garofalo, Roberto.

In: Journal of Immunology, Vol. 182, No. 5, 01.03.2009, p. 3072-3083.

Research output: Contribution to journalArticle

Guerrero-Plata, Antonieta ; Kolli, Deepthi ; Hong, Chao ; Casola, Antonella ; Garofalo, Roberto. / Subversion of pulmonary dendritic cell function by paramyxovirus infections. In: Journal of Immunology. 2009 ; Vol. 182, No. 5. pp. 3072-3083.
@article{f3e1b9ed2fc54314a75ba10bec2e995b,
title = "Subversion of pulmonary dendritic cell function by paramyxovirus infections",
abstract = "Lower respiratory tract infections caused by the paramyxoviruses human metapneumovirus (hMPV) and respiratory syncytial virus (RSV) are characterized by short-lasting virus-specific immunity and often long-term airway morbidity, both of which may be the result of alterations in the Ag-presenting function of the lung which follow these infections. In this study, we investigated whether hMPV and RSV experimental infections alter the phenotype and function of dendritic cell (DC) subsets that are recruited to the lung. Characterization of lung DC trafficking demonstrated a differential recruitment of plasmacytoid DC (pDC), conventional DC (cDC), and IFN-producing killer DC to the lung and draining lymph nodes after hMPV and RSV infection. In vitro infection of lung DC indicated that in pDC, production of IFN-α, TNF-α, and CCL5 was induced only by hMPV, whereas CCL3 and CCL4 were induced by both viruses. In cDC, a similar repertoire of cytokines was induced by hMPV and RSV, except for IFN-β, which was not induced by RSV. The function of lung pDC was altered following hMPV or RSV infection in vivo, as we demonstrated a reduced capacity of lung pDC to produce IFN-α as well as other cytokines including IL-6, TNF-α, CCL2, CCL3, and CCL4 in response to TLR9 stimulation. Moreover, we observed an impaired capacity of cDC from infected mice to present Ag to CD4+ T cells, an effect that lasted beyond the acute phase of infection. Our findings suggest that acute paramyxovirus infections can alter the long-term immune function of pulmonary DC.",
author = "Antonieta Guerrero-Plata and Deepthi Kolli and Chao Hong and Antonella Casola and Roberto Garofalo",
year = "2009",
month = "3",
day = "1",
doi = "10.4049/jimmunol.0802262",
language = "English (US)",
volume = "182",
pages = "3072--3083",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "5",

}

TY - JOUR

T1 - Subversion of pulmonary dendritic cell function by paramyxovirus infections

AU - Guerrero-Plata, Antonieta

AU - Kolli, Deepthi

AU - Hong, Chao

AU - Casola, Antonella

AU - Garofalo, Roberto

PY - 2009/3/1

Y1 - 2009/3/1

N2 - Lower respiratory tract infections caused by the paramyxoviruses human metapneumovirus (hMPV) and respiratory syncytial virus (RSV) are characterized by short-lasting virus-specific immunity and often long-term airway morbidity, both of which may be the result of alterations in the Ag-presenting function of the lung which follow these infections. In this study, we investigated whether hMPV and RSV experimental infections alter the phenotype and function of dendritic cell (DC) subsets that are recruited to the lung. Characterization of lung DC trafficking demonstrated a differential recruitment of plasmacytoid DC (pDC), conventional DC (cDC), and IFN-producing killer DC to the lung and draining lymph nodes after hMPV and RSV infection. In vitro infection of lung DC indicated that in pDC, production of IFN-α, TNF-α, and CCL5 was induced only by hMPV, whereas CCL3 and CCL4 were induced by both viruses. In cDC, a similar repertoire of cytokines was induced by hMPV and RSV, except for IFN-β, which was not induced by RSV. The function of lung pDC was altered following hMPV or RSV infection in vivo, as we demonstrated a reduced capacity of lung pDC to produce IFN-α as well as other cytokines including IL-6, TNF-α, CCL2, CCL3, and CCL4 in response to TLR9 stimulation. Moreover, we observed an impaired capacity of cDC from infected mice to present Ag to CD4+ T cells, an effect that lasted beyond the acute phase of infection. Our findings suggest that acute paramyxovirus infections can alter the long-term immune function of pulmonary DC.

AB - Lower respiratory tract infections caused by the paramyxoviruses human metapneumovirus (hMPV) and respiratory syncytial virus (RSV) are characterized by short-lasting virus-specific immunity and often long-term airway morbidity, both of which may be the result of alterations in the Ag-presenting function of the lung which follow these infections. In this study, we investigated whether hMPV and RSV experimental infections alter the phenotype and function of dendritic cell (DC) subsets that are recruited to the lung. Characterization of lung DC trafficking demonstrated a differential recruitment of plasmacytoid DC (pDC), conventional DC (cDC), and IFN-producing killer DC to the lung and draining lymph nodes after hMPV and RSV infection. In vitro infection of lung DC indicated that in pDC, production of IFN-α, TNF-α, and CCL5 was induced only by hMPV, whereas CCL3 and CCL4 were induced by both viruses. In cDC, a similar repertoire of cytokines was induced by hMPV and RSV, except for IFN-β, which was not induced by RSV. The function of lung pDC was altered following hMPV or RSV infection in vivo, as we demonstrated a reduced capacity of lung pDC to produce IFN-α as well as other cytokines including IL-6, TNF-α, CCL2, CCL3, and CCL4 in response to TLR9 stimulation. Moreover, we observed an impaired capacity of cDC from infected mice to present Ag to CD4+ T cells, an effect that lasted beyond the acute phase of infection. Our findings suggest that acute paramyxovirus infections can alter the long-term immune function of pulmonary DC.

UR - http://www.scopus.com/inward/record.url?scp=64849115293&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=64849115293&partnerID=8YFLogxK

U2 - 10.4049/jimmunol.0802262

DO - 10.4049/jimmunol.0802262

M3 - Article

VL - 182

SP - 3072

EP - 3083

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 5

ER -