Successful refolding and NMR structure of rMagi3: A disulfide-rich insecticidal spider toxin

Gustavo Titaux-Delgado, Elisa Carrillo, Angeles Mendoza, Marlen Mayorga-Flores, Fátima C. Escobedo-González, Patricia Cano-Sánchez, Estuardo López-Vera, Gerardo Corzo, Federico del Rio-Portilla

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The need for molecules with high specificity against noxious insects leads the search towards spider venoms that have evolved highly selective toxins for insect preys. In this respect, spiders as a highly diversified group of almost exclusive insect predators appear to possess infinite potential for the discovery of novel insect-selective toxins. In 2003, a group of toxins was isolated from the spider Macrothele gigas and the amino acid sequence was reported. We obtained, by molecular biology techniques in a heterologous system, one of these toxins. Purification process was optimized by chromatographic methods to determine the three-dimensional structure by nuclear magnetic resonance in solution, and, finally, their biological activity was tested. rMagi3 resulted to be a specific insect toxin with no effect on mice.

Original languageEnglish (US)
Pages (from-to)692-701
Number of pages10
JournalProtein Science
Volume27
Issue number3
DOIs
StatePublished - Mar 2018
Externally publishedYes

Keywords

  • Macrothele gigas
  • NMR solution structure
  • insecticidal
  • refolding disulfide-bonded toxins
  • spider toxin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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