Suckling and sucrose ingestion suppress persistent hyperalgesia and spinal fos expression after forepaw inflammation in infant rats

K. Ren, E. M. Blass, Q. Q. Zhou, R. Dubner

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Sweet taste and nonnutritive suckling produce analgesia to transient noxious stimuli in infant rats and humans. The present study evaluated the pain-modulating effects of sucrose and suckling in a rat model of persistent pain and hyperalgesia that mimics the response to tissue injury in humans. Fore- and hindpaw withdrawal latencies from a 30°or 48°C brass stylus were determined in 10-day-old rats following paw inflammation induced by complete Freund's adjuvant (CFA; 1:1 injected s.c, in a 0.01 ml volume). CFA markedly decreased escape latencies to both 48°and 30°C stimulation, thereby demonstrating thermal hyperalgesia and mechanical allodynia. The combination of nonnutritive suckling and sucrose (7.5%, 0.01-0.06 ml/min) infusion markedly increased escape latencies to forepaw stimulation in both CFA- treated and control rats. In contrast, intraoral sucrose and suckling did not increase hindpaw withdrawal latencies in either control or CFA-inflamed rats. The effect was specific to sweet taste because neither water nor isotonic saline infusion affected forepaw escape latencies. Parallel findings were obtained for CFA-induced Fos-like immunoreactivity (Fos-LI), a marker of neuronal activation. Fos-LI was selectively induced in cervical and lumbar regions ipsilateral to forepaw and hindpaw inflammation, respectively. Suckling-sucrose treatment significantly reduced Fos-LI at the cervical but not at the lumbar regions. These findings demonstrate: (i) the development of persistent pain and hyperalgesia in 10-day-old rats that can be attenuated by endogenous pain-modulating systems activated by taste and nonnutritive suckling; (ii) the mediation of the sucrose-suckling analgesia and antihyperalgesia at the spinal level; and (iii) a differential rostrocaudal maturation of descending pain-modulating systems to the spinal cord of 10- day-old rats. These findings may provide new clinical approaches for engaging endogenous analgesic mechanisms in infants following tissue injury and inflammation.

Original languageEnglish (US)
Pages (from-to)1471-1475
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number4
DOIs
StatePublished - Feb 18 1997

Fingerprint

Hyperalgesia
Sucrose
Eating
Inflammation
Pain
Lumbosacral Region
Analgesia
Freund's Adjuvant
Wounds and Injuries
Analgesics
Spinal Cord
Water

Keywords

  • analgesia
  • Freund's adjuvant
  • pain modulation

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Suckling and sucrose ingestion suppress persistent hyperalgesia and spinal fos expression after forepaw inflammation in infant rats. / Ren, K.; Blass, E. M.; Zhou, Q. Q.; Dubner, R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 4, 18.02.1997, p. 1471-1475.

Research output: Contribution to journalArticle

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