SUMO1 depletion prevents lipid droplet accumulation and HCV replication

Abdellah Akil, Ghaith Wedeh, Mohammad Zahid Mustafa, Ama Gassama-Diagne

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Infection by hepatitis C virus (HCV) is a major public-health problem. Chronic infection often leads to cirrhosis, steatosis, and hepatocellular carcinoma. The life cycle of HCV depends on the host cell machinery and involves intimate interaction between viral and host proteins. However, the role of host proteins in the life cycle of HCV remains poorly understood. Here, we identify the small ubiquitin-related modifier (SUMO1) as a key host factor required for HCV replication. We performed a series of cell biology and biochemistry experiments using the HCV JFH-1 (Japanese fulminate hepatitis 1) genotype 2a strain, which produces infectious particles and recapitulates all the steps of the HCV life cycle. We observed that SUMO1 is upregulated in Huh7.5 infected cells. Reciprocally, SUMO1 was found to regulate the expression of viral core protein. Moreover, knockdown of SUMO1 using specific siRNA influenced the accumulation of lipid droplets and reduced HCV replication as measured by qRT-PCR. Thus, we identify SUMO1 as a key host factor required for HCV replication. To our knowledge, this is the first report showing that SUMO1 regulates lipid droplets in the context of viral infection. Our report provides a meaningful insight into how HCV replicates and interacts with host proteins and is of significant importance for the field of HCV and RNA viruses.

Original languageEnglish (US)
Pages (from-to)141-148
Number of pages8
JournalArchives of Virology
Volume161
Issue number1
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Keywords

  • HCV
  • Lipid droplets
  • SUMO1

ASJC Scopus subject areas

  • Virology
  • Medicine(all)

Fingerprint Dive into the research topics of 'SUMO1 depletion prevents lipid droplet accumulation and HCV replication'. Together they form a unique fingerprint.

  • Cite this

    Akil, A., Wedeh, G., Zahid Mustafa, M., & Gassama-Diagne, A. (2016). SUMO1 depletion prevents lipid droplet accumulation and HCV replication. Archives of Virology, 161(1), 141-148. https://doi.org/10.1007/s00705-015-2628-3