15N-enriched 5-fluorocytosine as a probe for examining unusual DNA structures

Research output: Contribution to journalArticle

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Abstract

A new method is presented for the synthesis of oligonucleotides containing 15N-enriched 5-fluorocytosine (FC). Due to the reduced pK of FC, the amino protons of an unpaired FC residue may be observed at lower values of solution pH. The labeled FC residue has been placed as a template base at a model DNA replication fork. The amino protons of the FC residue have been identified in isotope-edited NMR spectra. Data is presented for a template FC residue unpaired, paired with guanine, and mispaired with adenine. These studies demonstrate the utility of labeled FC in examining unusual DNA structures.

Original languageEnglish (US)
Pages (from-to)713-723
Number of pages11
JournalJournal of Biomolecular Structure and Dynamics
Volume17
Issue number4
StatePublished - 2000
Externally publishedYes

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Flucytosine
Protons
DNA
Guanine
Adenine
DNA Replication
Oligonucleotides
Isotopes

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

15N-enriched 5-fluorocytosine as a probe for examining unusual DNA structures. / Sowers, Lawrence.

In: Journal of Biomolecular Structure and Dynamics, Vol. 17, No. 4, 2000, p. 713-723.

Research output: Contribution to journalArticle

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